Q5XC58 (GYRA_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	M6_Spy0870

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145266
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XC58 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 0CFCBF8AB137A623	FASTA	828	92,718
	10 20 30 40 50 60 MQDRNLIDVN LTSEMKTSFI DYAMSVIVAR ALPDVRDGLK PVHRRILYGM NELGVTPDKP 70 80 90 100 110 120 HKKSARITGD VMGKYHPHGD SSIYEAMVRM AQWWSYRHML VDGHGNFGSM DGDGAAAQRY 130 140 150 160 170 180 TEARMSKIAL ELLRDINKNT VNFQDNYDGS EREPVVLPAR FPNLLVNGAT GIAVGMATNI 190 200 210 220 230 240 PPHNLAESID AVKMVMEHPD CTTRELMEVI PGPDFPTGAL VMGRSGIHRA YDTGKGSIVL 250 260 270 280 290 300 RSRTEIETTQ TGRERIVVTE FPYGVNKTKV HEHIVRLAQE KRLEGITAVR DESSREGVRF 310 320 330 340 350 360 VIEIRREASA TVILNNLFKL TSLQTNFSFN MLAIENGVPK ILSLRQIIDN YISHQKEVII 370 380 390 400 410 420 RRTRFDKDKA EARAHILEGL LIALDHLDEV IAIIRNSETD VIAQTELMSR FDLSERQSQA 430 440 450 460 470 480 ILDMRLRRLT GLERDKIQSE YDDLLALIAD LSDILAKPER IITIIKEEMD EIKRKYANPR 490 500 510 520 530 540 RTELMVGEVL SLEDEDLIEE EDVLITLSNK GYIKRLAQDE FRAQKRGGRG VQGTGVNNDD 550 560 570 580 590 600 FVRELVSTST HDTLLFFTNF GRVYRLKAYE IPEYGRTAKG LPIVNLLKLE DGETIQTIIN 610 620 630 640 650 660 ARKEETAGKS FFFTTKQGIV KRTEVSEFNN IRQNGLRALK LKEGDQLINV LLTSGQDDII 670 680 690 700 710 720 IGTHSGYSVR FNEASIRNMG RSATGVRGVK LREDDRVVGA SRIRDNQEVL VITENGFGKR 730 740 750 760 770 780 TSATDYPTKG RGGKGIKTAN ITPKNGQLAG LVTVDGTEDI MVITNKGVII RTNVANISQT 790 800 810 820 GRATLGVKIM KLDADAKIVT FTLVQPEDSS IAEINTDREN SISKNKDN

References

[1]

"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87005.1.
RefSeq	YP_060188.1. NC_006086.1.
3D structure databases
ProteinModelPortal	Q5XC58.
SMR	Q5XC58. Positions 31-484.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000038241; EBSTRP00000036814; EBSTRG00000038239.
GeneID	2941381.
GenomeReviews	Gene locus M6_Spy0870 in contig CP000003_GR.
KEGG	spa:M6_Spy0870.
PATRIC	19723908. VBIStrPyo30273_0899.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000028052.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY0870-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STRP6

Accession

Primary (citable) accession number: Q5XC58

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program