Reviewed, UniProtKB/Swiss-Prot Q5XC12 (FTHS1_STRP6)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase 1
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase 1
      Short name=FHS 1
      Short name=FTHFS 1

Gene names

Name:	fhs1
Synonyms:	fhs, fthS
Ordered Locus Names:	M6_Spy0916

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.
Mass spectrometry	Molecular mass is 59531.12 Da from positions 1 - 556. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase 1 HAMAP MF_01543		PRO_0000199397
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XC12-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 90C6D132DF20EE3C	FASTA	556	59,531
	10 20 30 40 50 60 MKSDIEIAQS VALQPITDIV KKVGIDGDDI ELYGKYKAKL SFEKMKAVEA NEPGKLILVT 70 80 90 100 110 120 AINPTPAGEG KSTMSIGLAD ALNQMGKKTM LALREPSLGP VMGIKGGAAG GGYAQVLPME 130 140 150 160 170 180 DINLHFTGDM HAITTANNAL SALIDNHLQQ GNDLGIDPRR IIWKRVLDLN DRALRQVIVG 190 200 210 220 230 240 LGSPVNGVPR EDGFDITVAS EIMAILCLAT DLKDLKKRLA DIVVAYTYDR KPVYVRDLKV 250 260 270 280 290 300 EGALTLILKD AIKPNLVQTI YGTPALIHGG PFANIAHGCN SVLATSTALR LADYTVTEAG 310 320 330 340 350 360 FGADLGAEKF LNIKVPNLPK APDAIVIVAT LRALKMHGGV AKSDLAAENC EAVRLGFANL 370 380 390 400 410 420 KRHVENMRQF KVPVVVAINE FVADTEAEIA TLKALCEEIK VPVELASVWA NGAEGGLALA 430 440 450 460 470 480 KTVVRVIDQE AADYKRLYSD EDTLEEKVIN IVTQIYGGKA VQFGPKAKTQ LKQFAEFGWD 490 500 510 520 530 540 KLPVCMAKTQ YSFSDNPSLL GAPTDFDITI REFVPKTGAG FIVGLTGDVM TMPGLPKVPA 550 AMAMDVAENG TALGLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.
[2]	"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins." Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A. Submitted (MAY-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 3-20; 22-33; 54-87; 176-190; 321-331 AND 436-459, MASS SPECTROMETRY. Strain: JRS4 / Serotype M6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000003 Genomic DNA. Translation: AAT87051.1.
RefSeq	YP_060234.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2942562.
GenomeReviews	Gene locus M6_Spy0916 in contig CP000003_GR.
KEGG	spa:M6_Spy0916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5XC12.
OMA	GMKGGAT.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY0916-MON.
BRENDA	6.3.4.3. 314561.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS1_STRP6

Accession

Primary (citable) accession number: Q5XC12
Secondary accession number(s): P82572

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)