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Protein

Formate--tetrahydrofolate ligase 1

Gene

fhs1

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-976-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase 1 (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase 1
Short name:
FHS 1
Short name:
FTHFS 1
Gene namesi
Name:fhs1
Synonyms:fhs, fthS
Ordered Locus Names:M6_Spy0916
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001167 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligase 1PRO_0000199397Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XC12.
SMRiQ5XC12. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiEEPAHYT.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XC12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDIEIAQS VALQPITDIV KKVGIDGDDI ELYGKYKAKL SFEKMKAVEA
60 70 80 90 100
NEPGKLILVT AINPTPAGEG KSTMSIGLAD ALNQMGKKTM LALREPSLGP
110 120 130 140 150
VMGIKGGAAG GGYAQVLPME DINLHFTGDM HAITTANNAL SALIDNHLQQ
160 170 180 190 200
GNDLGIDPRR IIWKRVLDLN DRALRQVIVG LGSPVNGVPR EDGFDITVAS
210 220 230 240 250
EIMAILCLAT DLKDLKKRLA DIVVAYTYDR KPVYVRDLKV EGALTLILKD
260 270 280 290 300
AIKPNLVQTI YGTPALIHGG PFANIAHGCN SVLATSTALR LADYTVTEAG
310 320 330 340 350
FGADLGAEKF LNIKVPNLPK APDAIVIVAT LRALKMHGGV AKSDLAAENC
360 370 380 390 400
EAVRLGFANL KRHVENMRQF KVPVVVAINE FVADTEAEIA TLKALCEEIK
410 420 430 440 450
VPVELASVWA NGAEGGLALA KTVVRVIDQE AADYKRLYSD EDTLEEKVIN
460 470 480 490 500
IVTQIYGGKA VQFGPKAKTQ LKQFAEFGWD KLPVCMAKTQ YSFSDNPSLL
510 520 530 540 550
GAPTDFDITI REFVPKTGAG FIVGLTGDVM TMPGLPKVPA AMAMDVAENG

TALGLF
Length:556
Mass (Da):59,531
Last modified:November 23, 2004 - v1
Checksum:i90C6D132DF20EE3C
GO

Mass spectrometryi

Molecular mass is 59531.12 Da from positions 1 - 556. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT87051.1.
RefSeqiWP_002989724.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT87051; AAT87051; M6_Spy0916.
KEGGispa:M6_Spy0916.
PATRICi19724000. VBIStrPyo30273_0945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT87051.1.
RefSeqiWP_002989724.1. NC_006086.1.

3D structure databases

ProteinModelPortaliQ5XC12.
SMRiQ5XC12. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT87051; AAT87051; M6_Spy0916.
KEGGispa:M6_Spy0916.
PATRICi19724000. VBIStrPyo30273_0945.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiEEPAHYT.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSPYO286636:GHNO-976-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
    Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
    J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-946 / MGAS10394.
  2. "Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
    Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
    Submitted (MAY-2000) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 3-20; 22-33; 54-87; 176-190; 321-331 AND 436-459, MASS SPECTROMETRY.
    Strain: JRS4 / Serotype M6.

Entry informationi

Entry nameiFTHS1_STRP6
AccessioniPrimary (citable) accession number: Q5XC12
Secondary accession number(s): P82572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 23, 2004
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.