Q5XAQ3 (CYSK_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	cysK
Ordered Locus Names:	M6_Spy1375

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity. UniProtKB P47998
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2. UniProtKB P47998
Subunit structure	Homodimer By similarity. UniProtKB P47998
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 313	312	Cysteine synthase		PRO_0000259398
Regions
	Region	179 – 183	5	Pyridoxal phosphate binding By similarity UniProtKB P47998
	Region	215 – 220	6	SAT1 binding By similarity UniProtKB P47998
Sites
	Binding site	75	1	Pyridoxal phosphate By similarity UniProtKB P47998
	Binding site	267	1	Pyridoxal phosphate By similarity UniProtKB P47998
Amino acid modifications
	Modified residue	45	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XAQ3 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3FB5F881A0F669A3	FASTA	313	33,243
	10 20 30 40 50 60 MTKIYKTITE LVGQTPIIKL NRLIPNEAAD VYVKLEAFNP GSSVKDRIAL SMIEAAEAEG 70 80 90 100 110 120 LISPGDVIIE PTSGNTGIGL AWVGAAKGYR VIIVMPETMS LERRQIIQAY GAELVLTPGA 130 140 150 160 170 180 EGMKGAIAKT ETLAIELGAW MPMQFNNPAN PSIHEKTTAQ EILEAFKEIS LDAFVSGVGT 190 200 210 220 230 240 GGTLSGVSHV LKKASPETVI YAVEAEESAV LSGQEPGPHK IQGISAGFIP NTLDTKAYDQ 250 260 270 280 290 300 IIRVKSKDAL ETARLTGAKE GFLVGISSGA ALYAAIEVAK QLGKGKHVLT ILPDNGERYL 310 STELYDVPVI KTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.
[2]	"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins." Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A. Submitted (MAY-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-22; 91-124 AND 221-243. Strain: JRS4 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87510.1.
RefSeq	YP_060693.1. NC_006086.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z7W based on UniProtKB P47998.
ProteinModelPortal	Q5XAQ3.
SMR	Q5XAQ3. Positions 1-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000038858; EBSTRP00000037431; EBSTRG00000038856.
GeneID	2942124.
GenomeReviews	Gene locus M6_Spy1375 in contig CP000003_GR.
KEGG	spa:M6_Spy1375.
PATRIC	19724991. VBIStrPyo30273_1428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000028105.
HOGENOM	HBG748215.
OMA	RRNMIKA.
ProtClustDB	CLSK698791.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1375-MONOMER.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_STRP6

Accession

Primary (citable) accession number: Q5XAQ3
Secondary accession number(s): P82484

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program