Reviewed, UniProtKB/Swiss-Prot Q5XAQ1 (BPPI_STRP6)

Names and origin

Protein names

Recommended name:
    Putative bifunctional phosphatase/peptidyl-prolyl cis-trans isomerase
Including the following 2 domains:
    1- Recommended name:
            Phosphatase
              EC=3.1.3.-
    2- Recommended name:
            Peptidyl-prolyl cis-trans isomerase
                Short name=PPIase
                Short name=Rotamase
              EC=5.2.1.8

Gene names

Ordered Locus Names:

M6_Spy1377

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides By similarity. UniProtKB Q9H2H8
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). UniProtKB Q9H2H8
Cofactor	Magnesium By similarity. UniProtKB P0A8Y5
Sequence similarities	In the N-terminal section; belongs to the HAD-like hydrolase superfamily. Cof family. In the C-terminal section; belongs to the cyclophilin-type PPIase family. PPIL1 subfamily. Contains 1 PPIase cyclophilin-type domain.
Mass spectrometry	Molecular mass is 52146.29 Da from positions 1 - 470. Determined by ESI. Ref.2

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Isomerase Rotamase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Putative bifunctional phosphatase/peptidyl-prolyl cis-trans isomerase		PRO_0000308515
Regions
	Domain	286 – 468	183	PPIase cyclophilin-type
Sites
	Active site	22	1	Nucleophile By similarity UniProtKB P0A8Y5
	Metal binding	22	1	Magnesium By similarity UniProtKB P0A8Y5
	Metal binding	24	1	Magnesium By similarity UniProtKB P0A8Y5
	Metal binding	221	1	Magnesium By similarity UniProtKB P0A8Y5

Sequences

	Sequence		Length	Mass (Da)
	Q5XAQ1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 46386E388F193A5D	FASTA	470	52,223
	10 20 30 40 50 60 MEESMDAKLK YKAKKIKMVF FDIDDTLRVK DTGYMPESIQ RVFKALKAKG ILVGIASGRA 70 80 90 100 110 120 RYGVPQEVQD LHADYCVKLN GAYVKDDAKT IIFQAPIPAD VVVAYKKWAD DMGIFYGMAG 130 140 150 160 170 180 RHEAVLSARN DMISNAIDNV YAQLEVCPDY NEYHDVYQMW TFEDKGDGLQ LPAELAEHLR 190 200 210 220 230 240 LVRWHDNSSD VVLKGTSKAL GVSKVVDHLG LKPENILVFG DELNDLELFD YAGISIAMGV 250 260 270 280 290 300 SHPLLQEKAD FITKKVEEDG ILYALEELGL IDKELQFPQL DLENHTGPKV TIKTNHGDMT 310 320 330 340 350 360 LVLFPDHAPK TVANFLGLAK EGYYDGIIFH RIIPEFMIQG GDPTGTGMGG QSIYGESFED 370 380 390 400 410 420 EFSDELYNLR GALSMANAGP NTNGSQFFIV QNSKIPYAKK ELERGGWPTP IAAAYAENGG 430 440 450 460 470 TPHLDRRHTV FGQLVDETSF QVLDLIAGVE TGAQDKPKED VIIETIEVFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.
[2]	"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins." Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A. Submitted (MAY-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 294-331; 371-394 AND 428-458, MASS SPECTROMETRY. Strain: JRS4 / Serotype M6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000003 Genomic DNA. Translation: AAT87512.1.
RefSeq	YP_060695.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2942090.
GenomeReviews	Gene locus M6_Spy1377 in contig CP000003_GR.
KEGG	spa:M6_Spy1377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5XAQ1.
OMA	VLFPDHA.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1377-MON.
BRENDA	5.2.1.8. 314561.
Family and domain databases
InterPro	IPR013200. HAD-SF_hydro-like_3. IPR006379. HAD-SF_hydro_IIB. IPR000150. Hypothet_cof. IPR002130. PPIase_cyclophilin. [Graphical view]
Gene3D	G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.
Pfam	PF08282. Hydrolase_3. 1 hit. PF00160. Pro_isomerase. 1 hit. [Graphical view]
PRINTS	PR00153. CSAPPISMRASE.
TIGRFAMs	TIGR00099. Cof-subfamily. 1 hit. TIGR01484. HAD-SF-IIB. 1 hit.
PROSITE	PS01228. COF_1. False negative. PS01229. COF_2. False negative. PS00170. CSA_PPIASE_1. False negative. PS50072. CSA_PPIASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPPI_STRP6

Accession

Primary (citable) accession number: Q5XAQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)