Q5XAM5 (NADE_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	M6_Spy1403

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.
Sequence caution	The sequence AAT87538.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152210
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XAM5 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 4C9C45CEA938BCDB	FASTA	274	30,104
	10 20 30 40 50 60 MTLQEEIIRQ LGVKASIDPK EEIRKTVDFL KAYLRKHSFL KTYVLGISGG QDSTLAGKLA 70 80 90 100 110 120 QMAIAELREE TGDQAYQFIA VRLPYGVQAD EADAQKALAF IAPDQTLTIN IKAAVDGQVE 130 140 150 160 170 180 ALQAAGVEIS DFNKGNIKAR QRMISQYAIA GQMAGAVIGT DHAAENITGF FTKFGDGGAD 190 200 210 220 230 240 ILPLFRLNKR QGKALLKVLG ADAALYEKVP TADLEDQKPG LADEVALGVT YQDIDDYLEG 250 260 270 KLISKVAQAT IEKWWHKGQH KRHLPITIFD DFWK

References

[1]

"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87538.1. Different initiation.
RefSeq	YP_060721.1. NC_006086.1.
3D structure databases
ProteinModelPortal	Q5XAM5.
SMR	Q5XAM5. Positions 2-274.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000037154; EBSTRP00000035727; EBSTRG00000037154.
GeneID	2941201.
GenomeReviews	Gene locus M6_Spy1403 in contig CP000003_GR.
KEGG	spa:M6_Spy1403.
PATRIC	19725047. VBIStrPyo30273_1456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027750.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1403-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STRP6

Accession

Primary (citable) accession number: Q5XAM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program