Q5XAK9 (PROB_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	M6_Spy1419

Organism

Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) [Complete proteome] [HAMAP]

Taxonomic identifier

286636 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M6

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family.
Sequence caution	The sequence AAT87554.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109736
Regions
	Nucleotide binding	178 – 179	2	ATP By similarity
	Nucleotide binding	220 – 226	7	ATP By similarity
Sites
	Binding site	15	1	ATP By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	158	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XAK9 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 6B9FCC018B0008B6	FASTA	273	29,646
	10 20 30 40 50 60 MMKRQFEDVT RIVIKIGTSS LVLPTGKINL EKIDQLAFVI SSLMNKGKEV ILVSSGAMGF 70 80 90 100 110 120 GLDILKMEKR PTNLAKQQAV SSVGQVAMMS LYSQIFAHYQ TNVSQILLTR DVVVFPESLA 130 140 150 160 170 180 NVTNAFESLI SLGIVPIVNE NDAVSVDEMD HATKFGDNDR LSAVVAGITK ADLLIMLSDI 190 200 210 220 230 240 DGLFDKNPTI YEDAQLRSHV AVITQEIIAS AGGAGSKFGT GGMLSKVQSA QMVFENKGQM 250 260 270 VLMNGANPRD VLRVLEGQPL GTWFKQIEEV THD

References

[1]

"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87554.1. Different initiation.
RefSeq	YP_060737.1. NC_006086.1.
3D structure databases
ProteinModelPortal	Q5XAK9.
ModBase	Search...
Protein-protein interaction databases
STRING	286636.M6_Spy1419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT87554; AAT87554; M6_Spy1419.
GeneID	2941199.
KEGG	spa:M6_Spy1419.
PATRIC	19725079. VBIStrPyo30273_1472.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	VYTGNPK.
ProtClustDB	PRK12314.
Enzyme and pathway databases
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_STRP6

Accession

Primary (citable) accession number: Q5XAK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program