Reviewed, UniProtKB/Swiss-Prot Q5XAK0 (GLPO_STRP6)

Names and origin

Protein names

Recommended name:
    Alpha-glycerophosphate oxidase
    EC=1.1.3.21
Alternative name(s):
    Glycerol-3-phosphate oxidase

Gene names

Name:	glpO
Synonyms:	glpA
Ordered Locus Names:	M6_Spy1428

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Caution	As S.pyogenes is unable to produce acid from glycerol, the significance and/or function of the glpO gene in this organism is at present unknown.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Alpha-glycerophosphate oxidase		PRO_0000126112
Regions
	Nucleotide binding	21 – 49	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5XAK0-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 341CDF7964357A57	FASTA	612	67,914
	10 20 30 40 50 60 MEFSKETRRL ALQKMQERDL DLLIIGGGIT GAGVALQAAA SGLDTGLIEM QDFAEGTSSR 70 80 90 100 110 120 STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKP DPMLLPVYDE PGSTFSMFRL 130 140 150 160 170 180 KVAMDLYDLL AGVSNTPAAN KVLTKEEVLK REPDLKQEGL LGGGVYLDFR NNDARLVIEN 190 200 210 220 230 240 IKRANRDGAL IASHVKAEDF LLDDKRQIIG VKARDLLTDQ EIIIKAKLVI NTTGPWSDEI 250 260 270 280 290 300 RQFSHKGQPI HQMRPTKGVH LVVDRQKLPV SQPVYVDTGL NDGRMVFVLP REEKTYFGTT 310 320 330 340 350 360 DTDYTGDLQH PQVTQEDVDY LLGIVNNRFP NANLTINDIE SSWAGLRPLL SGNSASDYNG 370 380 390 400 410 420 GNSGKLSDDS FDHLIDTVKA YINHEDSREA VEKAIKQVET STSEKELDPS AVSRGSSFER 430 440 450 460 470 480 DENGLFTLAG GKITDYRKMA EGALKVIIQV LKEDFGKSFK LINSTTYPVS GGEINPANVD 490 500 510 520 530 540 SELEAYAQLG TLSGLSMDDA RYLANLYGSN APKVFALTRQ LKAAEGLSLA ETLSLHYAMD 550 560 570 580 590 600 YEMALKPTDY FLRRTNHLLF MRDSLDALIV PVIEEMAKHF DWSTDEKVKQ EEELRRVIAE 610 NDLSALKGQQ ED

References

[1]

"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87563.1.
RefSeq	YP_060746.1.
3D structure databases
SMR	Q5XAK0. Positions 3-608.
ModBase	Search...
Genome annotation databases
GeneID	2941714.
GenomeReviews	Gene locus M6_Spy1428 in contig CP000003_GR.
KEGG	spa:M6_Spy1428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG720894.
OMA	ASRDGQG.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1428-MONOMER.
BRENDA	1.1.3.21. 314561.
Family and domain databases
InterPro	IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_STRP6

Accession

Primary (citable) accession number: Q5XAK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)