Reviewed, UniProtKB/Swiss-Prot Q5X9Y7 (LACG_STRP6)

Names and originHide

Protein names

Recommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	M6_Spy1641

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributesHide

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

OntologiesHide

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260742
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	Q5X9Y7-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 36844A974A45B00D	FASTA	468	53,869
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 KLSEEFGVNG IRISIAWSRI FPTGKGEVNP KGVEYYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSNGDFL NRENIEHFVN YAELCFKEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLFKDSGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSDKTM EGVNHILEVN GGELDLREED FVALDAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWIIF PQGLYDQIMR 370 380 390 400 410 420 VKADYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKK HLEVISDAIS DGTNVKGYFM 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAYWYKK VAETQVIE

ReferencesHide

[1]

"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT87776.1.
RefSeq	YP_060959.1.
3D structure databases
HSSP	HSSP built from PDB template 2PBG based on UniProtKB P11546.
SMR	Q5X9Y7. Positions 1-468.
ModBase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000038532; EBSTRP00000037105; EBSTRG00000038530.
GeneID	2941307.
GenomeReviews	Gene locus M6_Spy1641 in contig CP000003_GR.
KEGG	spa:M6_Spy1641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG316462.
OMA	FCFKEFS.
ProtClustDB	PRK13511.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1641-MONOMER.
BRENDA	3.2.1.85. 314561.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

LACG_STRP6

Accession

Primary (citable) accession number: Q5X9Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 23, 2004
Last modified:	June 15, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)