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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathway:ilactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donorUniRule annotation
Active sitei375 – 3751NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-1721-MONOMER.
UniPathwayiUPA00542; UER00605.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
Ordered Locus Names:M6_Spy1641
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001167 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4684686-phospho-beta-galactosidasePRO_0000260742Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5X9Y7.
SMRiQ5X9Y7. Positions 1-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088632.
KOiK01220.
OMAiVERTQMG.
OrthoDBiEOG60658J.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5X9Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS
60 70 80 90 100
DFYNRYPVDL KLSEEFGVNG IRISIAWSRI FPTGKGEVNP KGVEYYHNLF
110 120 130 140 150
AECHKRHVEP FVTLHHFDTP EALHSNGDFL NRENIEHFVN YAELCFKEFS
160 170 180 190 200
EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY DLAKVFQSHH NMMVSHARAV
210 220 230 240 250
KLFKDSGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI IHNKFILDAT
260 270 280 290 300
YLGKYSDKTM EGVNHILEVN GGELDLREED FVALDAAKDL NDFLGINYYM
310 320 330 340 350
SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWIIF
360 370 380 390 400
PQGLYDQIMR VKADYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKK
410 420 430 440 450
HLEVISDAIS DGTNVKGYFM WSLMDVFSWS NGYEKRYGLF YVDFETQERY
460
PKKSAYWYKK VAETQVIE
Length:468
Mass (Da):53,869
Last modified:November 23, 2004 - v1
Checksum:i36844A974A45B00D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT87776.1.
RefSeqiWP_011184962.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT87776; AAT87776; M6_Spy1641.
KEGGispa:M6_Spy1641.
PATRICi19725564. VBIStrPyo30273_1706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT87776.1.
RefSeqiWP_011184962.1. NC_006086.1.

3D structure databases

ProteinModelPortaliQ5X9Y7.
SMRiQ5X9Y7. Positions 1-468.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT87776; AAT87776; M6_Spy1641.
KEGGispa:M6_Spy1641.
PATRICi19725564. VBIStrPyo30273_1706.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088632.
KOiK01220.
OMAiVERTQMG.
OrthoDBiEOG60658J.

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
BioCyciSPYO286636:GHNO-1721-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
    Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
    J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-946 / MGAS10394.

Entry informationi

Entry nameiLACG_STRP6
AccessioniPrimary (citable) accession number: Q5X9Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 23, 2004
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.