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Q5X9V1

- DEF_STRP6

UniProt

Q5X9V1 - DEF_STRP6

Protein

Peptide deformylase

Gene

def

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.By similarity

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

    Cofactori

    Binds 1 Fe2+ ion.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi131 – 1311IronBy similarity
    Metal bindingi174 – 1741IronBy similarity
    Active sitei175 – 1751By similarity
    Metal bindingi178 – 1781IronBy similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciSPYO286636:GHNO-1757-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylase (EC:3.5.1.88)
    Short name:
    PDF
    Alternative name(s):
    Polypeptide deformylase
    Gene namesi
    Name:def
    Ordered Locus Names:M6_Spy1677
    OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
    Taxonomic identifieri286636 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000001167: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Peptide deformylasePRO_0000082861Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi286636.M6_Spy1677.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5X9V1.
    SMRiQ5X9V1. Positions 2-204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0242.
    HOGENOMiHOG000243507.
    KOiK01462.
    OMAiHIDKENP.
    OrthoDBiEOG6PZXGQ.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5X9V1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAQDKLIKP SHLITMDDII REGNPTLRAV AKEVSLPLCD EDILLGEKMM    50
    QFLKHSQDPV MAEKLGLRAG VGLAAPQIDV SKRIIAVLVP NLPDKEGNPP 100
    KEAYSWQEVL YNPKIVSHSV QDAALSDGEG CLSVDRVVEG YVVRHARVTV 150
    DYYDKEGQQH RIKLKGYNAI VVQHEIDHIN GVLFYDRINA KNPFETKEEL 200
    LILD 204
    Length:204
    Mass (Da):22,862
    Last modified:November 23, 2004 - v1
    Checksum:i89F8EDE94D94DC05
    GO

    Mass spectrometryi

    Molecular mass is 22862.28 Da from positions 1 - 204. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000003 Genomic DNA. Translation: AAT87812.1.
    RefSeqiYP_060995.1. NC_006086.1.

    Genome annotation databases

    EnsemblBacteriaiAAT87812; AAT87812; M6_Spy1677.
    GeneIDi2941572.
    KEGGispa:M6_Spy1677.
    PATRICi19725638. VBIStrPyo30273_1743.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000003 Genomic DNA. Translation: AAT87812.1 .
    RefSeqi YP_060995.1. NC_006086.1.

    3D structure databases

    ProteinModelPortali Q5X9V1.
    SMRi Q5X9V1. Positions 2-204.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 286636.M6_Spy1677.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT87812 ; AAT87812 ; M6_Spy1677 .
    GeneIDi 2941572.
    KEGGi spa:M6_Spy1677.
    PATRICi 19725638. VBIStrPyo30273_1743.

    Phylogenomic databases

    eggNOGi COG0242.
    HOGENOMi HOG000243507.
    KOi K01462.
    OMAi HIDKENP.
    OrthoDBi EOG6PZXGQ.

    Enzyme and pathway databases

    BioCyci SPYO286636:GHNO-1757-MONOMER.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
      Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
      J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-946 / MGAS10394.
    2. "Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
      Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
      Submitted (MAY-2000) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 7-20; 68-101; 148-161; 166-187 AND 192-204, MASS SPECTROMETRY.
      Strain: JRS4 / Serotype M6.

    Entry informationi

    Entry nameiDEF_STRP6
    AccessioniPrimary (citable) accession number: Q5X9V1
    Secondary accession number(s): P82590
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3