Q5X9A9 (HASA_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan synthase
EC=2.4.1.212

Alternative name(s):
Hyaluronate synthase
Hyaluronic acid synthase
Short name=HA synthase

Gene names

Name:	hasA
Ordered Locus Names:	M6_Spy1869

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycosaminoglycan synthesis. The hyaluronic acid capsule is involved in the pathogenicity of group A Streptococci; it may be the major virulence determinant.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the nodC/HAS family.
Caution	It is uncertain whether Met-1 or Met-25 is the initiator.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Virulence
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Hyaluronan synthase		PRO_0000197167
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Transmembrane	345 – 365	21	Helical; Potential
	Transmembrane	376 – 396	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5X9A9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 803949A754A89FF8	FASTA	419	47,886
	10 20 30 40 50 60 MPIFKKTLIV LSFIFLISIL IYLNMYLFGT STVGIYGVIL ITYLVIKLGL SFLYEPFKGN 70 80 90 100 110 120 PHDYKVAAVI PSYNEDAESL LETLKSVLAQ TYPLSEIYIV DDGSSNTDAI QLIEEYVNRE 130 140 150 160 170 180 VDICRNVIVH RSLVNKGKRH AQAWAFERSD ADVFLTVDSD TYIYPNALEE LLKSFNDETV 190 200 210 220 230 240 YAATGHLNAR NRQTNLLTRL TDIRYDNAFG VERAAQSLTG NILVCSGPLS IYRREVIIPN 250 260 270 280 290 300 LERYKNQTFL GLPVSIGDDR CLTNYAIDLG RTVYQSTARC DTDVPFQLKS YLKQQNRWNK 310 320 330 340 350 360 SFFRESIISV KKILSNPIVA LWTIFEVVMF MMLIVAIGNL LFNQAIQLDL IKLFAFLSII 370 380 390 400 410 FIVALCRNVH YMVKHPASFL LSPLYGILHL FVLQPLKLYS LCTIKNTEWG TRKKVTIFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes." DeAngelis P.L., Papaconstantinou J., Weigel P.H. J. Biol. Chem. 268:19181-19184(1993) [PubMed: 8366070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 21547 / S43 / Serotype M6.
[2]	"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20853 Unassigned DNA. Translation: AAA17981.1. L20853 Unassigned DNA. Translation: AAA17984.1. CP000003 Genomic DNA. Translation: AAT88004.1.
RefSeq	YP_061187.1. NC_006086.1.
3D structure databases
ProteinModelPortal	Q5X9A9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000037935; EBSTRP00000036508; EBSTRG00000037933.
GeneID	2940686.
GenomeReviews	Gene locus M6_Spy1869 in contig CP000003_GR.
KEGG	spa:M6_Spy1869.
PATRIC	19726043. VBIStrPyo30273_1943.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000026831.
HOGENOM	HBG339192.
OMA	WYHQTFL.
ProtClustDB	CLSK687618.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY1869-MONOMER.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
KO	K00752.
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HASA_STRP6

Accession

Primary (citable) accession number: Q5X9A9
Secondary accession number(s): Q08494, Q54865, Q54868

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program