Reviewed, UniProtKB/Swiss-Prot Q5X8J8 (KATG2_LEGPA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	lpp0252

Organism

Legionella pneumophila (strain Paris) [Complete proteome] [HAMAP]

Taxonomic identifier

297246 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 749	722	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354821
Sites
	Active site	108	1	Proton acceptor By similarity
	Metal binding	270	1	Iron (heme axial ligand) By similarity
	Site	104	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	107 ↔ 229		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255) By similarity
	Cross-link	229 ↔ 255		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5X8J8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4665876E0BA2FE15	FASTA	749	82,988
	10 20 30 40 50 60 MFKRTIPLFA AFTLAISPSV FPNYAYAQED KPKTNQYWWP KMLDLSPLRQ PNATSNPMGE 70 80 90 100 110 120 QFNYAEEFNS LDLNAVIEDL KKLMTTSQDW WPADYGNYGP LFIRMSWHAA GTYRIYDGRG 130 140 150 160 170 180 GANGGFQRFA PQNSWPDNAN LDKARRLLWP IKQKYGRKIS WADLLVLAGN VAMESMGFKT 190 200 210 220 230 240 IGFAGGREDA WEAININWGP EGKWLESKRQ DKDGKLEKPL AATVMGLIYV NPEGPNGVPD 250 260 270 280 290 300 PLAAAEKIRE TFGRMAMNDE ETVALIAGGH AFGKTHGAAS GKYLGPAPEA AGIEEQGFGW 310 320 330 340 350 360 KNSYGSGKGK DTITSGLEGA WTVTPTHWSH NYLQNLFNFN WVKTKSPGGA IQWVPENSNA 370 380 390 400 410 420 SSMVPDAFDP SKRHAPVMLT TDLALKFDPV YSKIAKRFLD NPKEFDDAFA RAWFKLIHRD 430 440 450 460 470 480 MGPRSRYLGS LVPKEIMIWQ DPVPPVDYKL VDANDIANLK GKILNSGLST SELVKTAWAS 490 500 510 520 530 540 ASTFRGTDMR GGANGARIRL SPQKDWPAND PQELAKVLKT LESIQNNFNN AQADGKKISL 550 560 570 580 590 600 ADLIVLGGNA AIEQAAKQAG YDIIVPFMPG RTDATQGMTD VKSFEVLEPK ADGFRNYFDK 610 620 630 640 650 660 SNNMSPPEML VEKASLLKLS VPQMTVLVGG MRVLNANTGQ NQYGVFTDKP GTLNNDFFVN 670 680 690 700 710 720 LLSMSTEWKK SSETEGIYEG YDRKTGKLKW KATSVDLIFG ANSELRAVAE AYATDDAKEK 730 740 FIQDFVNAWV KVMTADRFDI KAANTNINS

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed: 15467720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR628336 Genomic DNA. Translation: CAH11399.1.
RefSeq	YP_122595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5X8J8.
Protein family/group databases
PeroxiBase	2398. LpnCP01_Paris.
Genome annotation databases
GeneID	3117738.
GenomeReviews	Gene locus lpp0252 in contig CR628336_GR.
KEGG	lpp:lpp0252.
Organism-specific databases
LegioList	lpp0252.
CMR	Search...
Phylogenomic databases
HOGENOM	Q5X8J8.
OMA	GTDMRGG.
Enzyme and pathway databases
BioCyc	LPNE297246:LPP0252-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_LEGPA

Accession

Primary (citable) accession number: Q5X8J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)