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Q5X6G6

- SYI_LEGPA

UniProt

Q5X6G6 - SYI_LEGPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Legionella pneumophila (strain Paris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi894 – 8941ZincUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi914 – 9141ZincUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLPNE297246:GCO9-1278-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:lpp0999
    OrganismiLegionella pneumophila (strain Paris)
    Taxonomic identifieri297246 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
    ProteomesiUP000000610: Chromosome

    Organism-specific databases

    LegioListilpp0999.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 931931Isoleucine--tRNA ligasePRO_0000098406Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi297246.lpp0999.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5X6G6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5X6G6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEYKDTLNL PNTSFPMKAS LSVREPEMLA DWQAKGIYQK IRKARVGSKR    50
    FILHDGPPYA NGHLHCGHAL NKILKDIIIK SKTFSGFDAP FVPGWDCHGL 100
    PIELNVEKKV GKAGSKISPR EFRAKCREYA ASQIDIQRDE FQRLGVLGDW 150
    YNPYVTMDYH YEANIVRALG LMIKNGHLQQ GFKPVHWCID CGSALAEAEV 200
    DYEDKTSPSI DVAFSAVNPS EFLNCFETQP AVKPLILPIW TTTPWTLPAN 250
    EAVCLHPEID YALIDAGNSY YIVATDLVES VMARYGISHY KTSGSAKGRV 300
    FEHFKLQHPF YKRQVPVVLA EHVTTESGTG CVHTAPAHGP DDYLVGQSYQ 350
    LPLINPVMAN GCFAENVELF AGISVLKANE TILAVLSERN VLLASESIRH 400
    SYPHCWRHKS PMIFLATPQW FISMDKSNLR QAIINEIDKV NWVPDWGKAR 450
    ISNMVENRPD WCISRQRSWG TPMPLFVHKT TRELHPDTLE LIERVAVMIE 500
    KSGIDAWFDL DSSELLGDDA KHYDKITDTM DVWLDSGISH YSVLKHNNDL 550
    DFPADVYFEG SDQHRGWFNS SLTTAVAMYG VAPYKTVLTH GYTVDAEGKK 600
    LSKSKGNYVA LDKLVNQHGA DILRLWVAST DYRHEVSISE EIIKRNADAY 650
    RRIRNTARFL LANLFDFNPA SDCIEAKELL ELDRWALKRC QLLQEEIITA 700
    YENYHFHLIY QKIHNFCAVD MGSFYLDLIK DRQYTTAKDS IARRSCQTAM 750
    YHMVKAFTIW LAPILSFTAE EIWQTIPGNN SESIFIEHWY DAWPIIDAVN 800
    MEDWEQLHIV RDEVNKALEE TRQRGEIGSA LAAEVTVYAD AKALPKLTRL 850
    GEELRFLFIT SEAKACPISQ SPKGLAVTDC GVSIQVTASA HEKCARCWHR 900
    REDVGQNQEH PELCLRCVGN ISGYHEERLY I 931
    Length:931
    Mass (Da):105,744
    Last modified:November 23, 2004 - v1
    Checksum:i77B18BB6C8C04521
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR628336 Genomic DNA. Translation: CAH12150.1.
    RefSeqiYP_123327.1. NC_006368.1.

    Genome annotation databases

    EnsemblBacteriaiCAH12150; CAH12150; lpp0999.
    GeneIDi3118229.
    KEGGilpp:lpp0999.
    PATRICi22322301. VBILegPne27771_1213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR628336 Genomic DNA. Translation: CAH12150.1 .
    RefSeqi YP_123327.1. NC_006368.1.

    3D structure databases

    ProteinModelPortali Q5X6G6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 297246.lpp0999.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH12150 ; CAH12150 ; lpp0999 .
    GeneIDi 3118229.
    KEGGi lpp:lpp0999.
    PATRICi 22322301. VBILegPne27771_1213.

    Organism-specific databases

    LegioListi lpp0999.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LPNE297246:GCO9-1278-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity."
      Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C.
      Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Paris.

    Entry informationi

    Entry nameiSYI_LEGPA
    AccessioniPrimary (citable) accession number: Q5X6G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3