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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Legionella pneumophila (strain Paris)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLPNE297246:GCO9-1662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:lpp1302
OrganismiLegionella pneumophila (strain Paris)
Taxonomic identifieri297246 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000000610: Chromosome

Organism-specific databases

LegioListilpp1302.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823Leucine--tRNA ligasePRO_0000152031Add
BLAST

Proteomic databases

PRIDEiQ5X5L8.

Interactioni

Protein-protein interaction databases

STRINGi297246.lpp1302.

Structurei

3D structure databases

ProteinModelPortaliQ5X5L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5X5L8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNTYNPQEV EEQAQQYWHK KQSFNVTEDL NKEKFYCLSM FPYPSGTLHM
60 70 80 90 100
GHVRNYTLGD VIARYQRALG KNVLQPIGWD AFGLPAENAA IKNKIPPAEW
110 120 130 140 150
TRKNIAAMKE QFLRLGNAYD WKREITTCDP EYYRWEQWFF IRLFEKGLVY
160 170 180 190 200
KKNAVVNWDP VDQTVLANEQ VVDGRGWRSG ALVERKEISQ WFIKITSYAD
210 220 230 240 250
ELLSSLDSLD EWPAQVKQMQ RNWIGKSIGT EIYFNVNNYP KRLKIYTTRP
260 270 280 290 300
DTLMGATYLA VATDHPLAKE AASNNKKVQE FLDSCQGIKI AEAELATMEK
310 320 330 340 350
RGIDTGMTAI HPITGKELPI WVANFVLMQY GSGAVMAVPA HDQRDWEFAQ
360 370 380 390 400
KYQLPVKQVI KPIDIEHDFN QSAYTEEGIL INSNQFDNLL SSKAIQVITN
410 420 430 440 450
FLEENDAGKA TINYRLRDWG VSRQRYWGTP IPMIICEQCG IVPVPDEELP
460 470 480 490 500
VVLPENVDFT GTGSPLTQCK EFVNVTCPKC GQDANRETDT FDTFVESSWY
510 520 530 540 550
YARFACKGQE NAMLDDRAKY WTPVDQYIGG IEHAVMHLLY ARFFHKLMRD
560 570 580 590 600
EGLVNSDEPF KALLTQGMVL KDGHKMSKSL GNVVDPNHLI NTYGADTARL
610 620 630 640 650
FVMFASPPEQ SLEWSDSGVE GAHRFLKRVW AFSHQHRDML IDINDSILSG
660 670 680 690 700
NGHVDWKEAE SRLKKSRHIV HQILAQATHD YDRNQFNTVV SGCMKLFNEI
710 720 730 740 750
SDYSIETEND KFFIHSSISI LLRLLAPITP HICHCLWQQL GFDKAIIDAP
760 770 780 790 800
WPKVDKSALK TDEVDYVVQV NGKLRAQFTA STDASEEELI AAAKEHAHNF
810 820
VVNHTIKKAI IVPHRQLINL VIG
Length:823
Mass (Da):94,105
Last modified:November 23, 2004 - v1
Checksum:i53069F3A8F74E132
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR628336 Genomic DNA. Translation: CAH12453.1.
RefSeqiYP_123626.1. NC_006368.1.

Genome annotation databases

EnsemblBacteriaiCAH12453; CAH12453; lpp1302.
GeneIDi3117453.
KEGGilpp:lpp1302.
PATRICi22322971. VBILegPne27771_1544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR628336 Genomic DNA. Translation: CAH12453.1.
RefSeqiYP_123626.1. NC_006368.1.

3D structure databases

ProteinModelPortaliQ5X5L8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi297246.lpp1302.

Proteomic databases

PRIDEiQ5X5L8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH12453; CAH12453; lpp1302.
GeneIDi3117453.
KEGGilpp:lpp1302.
PATRICi22322971. VBILegPne27771_1544.

Organism-specific databases

LegioListilpp1302.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLPNE297246:GCO9-1662-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity."
    Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C.
    Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Paris.

Entry informationi

Entry nameiSYL_LEGPA
AccessioniPrimary (citable) accession number: Q5X5L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.