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Q5X5L8 (SYL_LEGPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:lpp1302
OrganismLegionella pneumophila (strain Paris) [Complete proteome] [HAMAP]
Taxonomic identifier297246 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella

Protein attributes

Sequence length823 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 823823Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152031

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5X5L8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 53069F3A8F74E132

FASTA82394,105
        10         20         30         40         50         60 
MDNTYNPQEV EEQAQQYWHK KQSFNVTEDL NKEKFYCLSM FPYPSGTLHM GHVRNYTLGD 

        70         80         90        100        110        120 
VIARYQRALG KNVLQPIGWD AFGLPAENAA IKNKIPPAEW TRKNIAAMKE QFLRLGNAYD 

       130        140        150        160        170        180 
WKREITTCDP EYYRWEQWFF IRLFEKGLVY KKNAVVNWDP VDQTVLANEQ VVDGRGWRSG 

       190        200        210        220        230        240 
ALVERKEISQ WFIKITSYAD ELLSSLDSLD EWPAQVKQMQ RNWIGKSIGT EIYFNVNNYP 

       250        260        270        280        290        300 
KRLKIYTTRP DTLMGATYLA VATDHPLAKE AASNNKKVQE FLDSCQGIKI AEAELATMEK 

       310        320        330        340        350        360 
RGIDTGMTAI HPITGKELPI WVANFVLMQY GSGAVMAVPA HDQRDWEFAQ KYQLPVKQVI 

       370        380        390        400        410        420 
KPIDIEHDFN QSAYTEEGIL INSNQFDNLL SSKAIQVITN FLEENDAGKA TINYRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGTP IPMIICEQCG IVPVPDEELP VVLPENVDFT GTGSPLTQCK EFVNVTCPKC 

       490        500        510        520        530        540 
GQDANRETDT FDTFVESSWY YARFACKGQE NAMLDDRAKY WTPVDQYIGG IEHAVMHLLY 

       550        560        570        580        590        600 
ARFFHKLMRD EGLVNSDEPF KALLTQGMVL KDGHKMSKSL GNVVDPNHLI NTYGADTARL 

       610        620        630        640        650        660 
FVMFASPPEQ SLEWSDSGVE GAHRFLKRVW AFSHQHRDML IDINDSILSG NGHVDWKEAE 

       670        680        690        700        710        720 
SRLKKSRHIV HQILAQATHD YDRNQFNTVV SGCMKLFNEI SDYSIETEND KFFIHSSISI 

       730        740        750        760        770        780 
LLRLLAPITP HICHCLWQQL GFDKAIIDAP WPKVDKSALK TDEVDYVVQV NGKLRAQFTA 

       790        800        810        820 
STDASEEELI AAAKEHAHNF VVNHTIKKAI IVPHRQLINL VIG 

« Hide

References

[1]"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity."
Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C.
Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Paris.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR628336 Genomic DNA. Translation: CAH12453.1.
RefSeqYP_123626.1. NC_006368.1.

3D structure databases

ProteinModelPortalQ5X5L8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING297246.lpp1302.

Proteomic databases

PRIDEQ5X5L8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH12453; CAH12453; lpp1302.
GeneID3117453.
KEGGlpp:lpp1302.
PATRIC22322971. VBILegPne27771_1544.

Organism-specific databases

LegioListlpp1302.
CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLPNE297246:GCO9-1662-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEGPA
AccessionPrimary (citable) accession number: Q5X5L8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries