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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Legionella pneumophila (strain Paris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Magnesium or manganese 1UniRule annotation1
Metal bindingi314Magnesium or manganese 1UniRule annotation1
Metal bindingi314Magnesium or manganese 2UniRule annotation1
Metal bindingi316Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 222ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:lpp2665
OrganismiLegionella pneumophila (strain Paris)
Taxonomic identifieri297246 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella

Organism-specific databases

LegioListilpp2665

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000304581 – 364D-alanine--D-alanine ligaseAdd BLAST364

Proteomic databases

PRIDEiQ5X1S8

Structurei

3D structure databases

ProteinModelPortaliQ5X1S8
SMRiQ5X1S8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 347ATP-graspUniRule annotationAdd BLAST214

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiRCDFFVI
OrthoDBiPOG091H06GK

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5X1S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPVNLVLL YGGKSGEHEV SLVSAASVLK HLDSEKYHII PIAMDKSGRF
60 70 80 90 100
HRHDYNDLLA CSDKLPVVTE KSTPLEGLLI NGRLAVDAEI VFPVVHGPLY
110 120 130 140 150
EDGCLQGLLE LAGVAYVGCD VLSSAIGMDK DMARRLACIN GLKSARYKLL
160 170 180 190 200
SWHANASERQ QFCHEVASEF GWPLFVKPCS LGSSVGIHKA NNMDELNAAV
210 220 230 240 250
ADALRYDEEI LVEEFIVGRE IELAVLENSI PCGKPRVSMV GEIKVNHPDG
260 270 280 290 300
YYSYTAKYLE SSQTDLIIPA QLNNSLEEQL KQAAANIFSY LKCKGMARVD
310 320 330 340 350
FFVNDKTEEI YFNEINTLPG FTSISMYPKL WQATGVAYPD LLDELINLAM
360
VHHNCRQHLV TNYL
Length:364
Mass (Da):40,409
Last modified:November 23, 2004 - v1
Checksum:iDA13815F5B3EF5A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR628336 Genomic DNA Translation: CAH13818.1

Genome annotation databases

EnsemblBacteriaiCAH13818; CAH13818; lpp2665
KEGGilpp:lpp2665

Similar proteinsi

Entry informationi

Entry nameiDDL_LEGPA
AccessioniPrimary (citable) accession number: Q5X1S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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