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Q5WXX1

- SYI_LEGPL

UniProt

Q5WXX1 - SYI_LEGPL

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Protein

Isoleucine--tRNA ligase

Gene
ileS, lpl0968
Organism
Legionella pneumophila (strain Lens)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi894 – 8941Zinc By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLPNE297245:GJD4-1035-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:lpl0968
OrganismiLegionella pneumophila (strain Lens)
Taxonomic identifieri297245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000002517: Chromosome

Organism-specific databases

LegioListilpl0968.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligaseUniRule annotationPRO_0000098405Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi297245.lpl0968.

Structurei

3D structure databases

ProteinModelPortaliQ5WXX1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5WXX1-1 [UniParc]FASTAAdd to Basket

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MAEYKDTLNL PNTSFPMKAS LSVREPEMLA DWQAKGIYQK IRKARVGSKR    50
FILHDGPPYA NGHLHCGHAL NKILKDIIIK SKTFSGFDAP FVPGWDCHGL 100
PIELNVEKKV GKAGSKISPR EFRAKCREYA ASQIDIQRDE FQRLGVLGDW 150
YNPYVTMDYH YEANIVRALG LMIKNGHLQQ GFKPVHWCID CGSALAEAEV 200
DYEDKTSPSI DVAFSAVNPS EFLNCFGNQL AVKPLILPIW TTTPWTLPAN 250
EAVCLHPEID YALIDADNSY YIVATDLVES VMARYGISHY KTSGSAKGRV 300
FEHFKLQHPF YKRQVPVVLA EHVTTESGTG SVHTAPAHGP DDYLVGQFYQ 350
LPLINPVMAN GCFAENVELF AGISVLKANE TILAVLSERN VLLASESIRH 400
SYPHCWRHKS PMIFLATPQW FISMDKSNLR QAIINEIDKV NWVPDWGKAR 450
ISNMVENRPD WCISRQRSWG TPMPLFVHKT TRELHPDTLE LIEKVAVMIE 500
KSGIDAWFDL DSSELLGDDA KHYDKITDTM DVWLDSGISH YSVLKHNNDL 550
DFPADVYFEG SDQHRGWFNS SLTTAVAMYG VAPYKTVLTH GYTVDAEGKK 600
LSKSKGNYVA LDKLVNQHGA DILRLWVAST DYRHEVSISE EIIKRNADAY 650
RRIRNTARFL LANLFDFNPA SDCIDAKELL ELDRWALKRC QLLQEEIITA 700
YDNYHFHLIY QKIHNFCAVD MGSFYLDLIK DRQYTTAKDS IARRSCQTAM 750
YHMVKAFTIW LAPILSFTAE EIWQTIPGNN SESIFIEHWY DAWPTIDAVN 800
MEDWEQLHIV RDEVNKALEE TRQRGEIGSA LAAEVTVYAD AKALPKLTRL 850
GEELRFLFIT SEAKACPISQ SPKGLAVTDC GVSIQVTASA HEKCARCWHR 900
REDVGQNQEH PELCLRCVGN ISGYHEERLY I 931
Length:931
Mass (Da):105,735
Last modified:November 23, 2004 - v1
Checksum:iA89611DB7D84235F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR628337 Genomic DNA. Translation: CAH15202.1.
RefSeqiYP_126327.1. NC_006369.1.

Genome annotation databases

EnsemblBacteriaiCAH15202; CAH15202; lpl0968.
GeneIDi3114030.
KEGGilpf:lpl0968.
PATRICi22315519. VBILegPne33733_1092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR628337 Genomic DNA. Translation: CAH15202.1 .
RefSeqi YP_126327.1. NC_006369.1.

3D structure databases

ProteinModelPortali Q5WXX1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 297245.lpl0968.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH15202 ; CAH15202 ; lpl0968 .
GeneIDi 3114030.
KEGGi lpf:lpl0968.
PATRICi 22315519. VBILegPne33733_1092.

Organism-specific databases

LegioListi lpl0968.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LPNE297245:GJD4-1035-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity."
    Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C.
    Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Lens.

Entry informationi

Entry nameiSYI_LEGPL
AccessioniPrimary (citable) accession number: Q5WXX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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