Reviewed, UniProtKB/Swiss-Prot Q5WVZ4 (ASTD_LEGPL)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	lpl1666

Organism

Legionella pneumophila (strain Lens) [Complete proteome] [HAMAP]

Taxonomic identifier

297245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262405
Regions
	Nucleotide binding	228 – 233	6	NAD By similarity
Sites
	Active site	251	1	By similarity
	Active site	285	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WVZ4-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DF2B04954B45501C	FASTA	495	54,521
	10 20 30 40 50 60 MSKLQIIQSK GQYINGEWIK GNGLILESTN PASGTLLWQG NNATDEEIAN ACYVAHRALK 70 80 90 100 110 120 SWANTSFEER ARYTKAFVEQ VEKNRDQLAR LISLETGKPL WESQTEVSSV IGKVNLSIQA 130 140 150 160 170 180 YQERTWPKQT ETAEANACLR FKPHGVVVVL GAFNFPAHLS NGHIVPALLA GNTVLYKPSE 190 200 210 220 230 240 HTPAVAELII QCWHDSGLPP GVINCLQGNA NCGNTLLSQD IQGVYFTGSY ATGLRIHQQF 250 260 270 280 290 300 CNRPEIILAL EMGGNNPLVI DEVKDIDAAV YHTMLSTMIT AGQRCTCARR IIVPDSQTGD 310 320 330 340 350 360 LFLERFAKAC KLMRIGSFDS QPEPFIGPVI SHVQALKHLH AQKQLVEMGG EIILPMSLLV 370 380 390 400 410 420 EYTGLISPGI IDMTRAKNPP DEEIFAPFAQ IYRYNHFDEA IQLANQTRYG LSAGLLSDNK 430 440 450 460 470 480 DHYLQFYQHI RAGLINWNRP TTGAASSLPF GGVGCSGNHR PSAYFAADYC AYPVASMEQP 490 LLTTPAQRLP GLVLE

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed: 15467720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR628337 Genomic DNA. Translation: CAH15906.1.
RefSeq	YP_127005.1.
3D structure databases
SMR	Q5WVZ4. Positions 10-439, 12-465.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WVZ4.
Genome annotation databases
GeneID	3113691.
GenomeReviews	Gene locus lpl1666 in contig CR628337_GR.
KEGG	lpf:lpl1666.
NMPDR	fig|297245.3.peg.1848.
Organism-specific databases
LegioList	lpl1666.
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	KAYHART.
PhylomeDB	Q5WVZ4.
Enzyme and pathway databases
BioCyc	LPNE297245:LPL1666-MONOMER.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_LEGPL

Accession

Primary (citable) accession number: Q5WVZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)