Q5WU58 (KATG1_LEGPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	lpl2313

Organism

Legionella pneumophila (strain Lens) [Complete proteome] [HAMAP]

Taxonomic identifier

297245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354818
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WU58 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F589328140AD8C6C	FASTA	721	80,521
	10 20 30 40 50 60 MDGKVGNTAT GCPVMHGGMT SAETSNTAWW PNALNLDILH QHDTKTNPMG KNFNYREEVK 70 80 90 100 110 120 KLDFEALKKD LHALMTDSQP WWPADWGHYG GLMIRMSWHA AGSYRVADGR GGAGTGNQRF 130 140 150 160 170 180 APLNSWPDNV NLDKARRLLW PIKKKYGNKI SWADLIALAG TIAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDVYW GSEQEWLGAK RYDGKSRESL ENPLAAVQMG LIYVNPEGVN GQPDPLRTAQ 250 260 270 280 290 300 DVRVTFGRMA MNDEETVALT AGGHTVGKCH GNGNAKFLGP DPEAADVEDQ GLGWINKTTR 310 320 330 340 350 360 GIGRNTVSSG IEGAWTTHPT QWDNGYFYLL LNYDWELKKS PAGAWQWEPI HIKEEDKPVD 370 380 390 400 410 420 VEDPAIRRNP IMTDADMAIK MDPVYRKIAE RFYKDPDYFA EVFARAWFKL THRDMGPKTR 430 440 450 460 470 480 YIGPDVPKED LIWQDPVPAG NRAYDIAAAK AKIAASNLTI GEMVSTAWDS ARTFRGSDKR 490 500 510 520 530 540 GGANGARIRL KPQKDWEGNE PQRLTKVLRI LESIAADTGA SVADVIVLAG NVGIEKAAKA 550 560 570 580 590 600 AGFDIIVPFA PGRGDATDDM TDAESFDVLE PLHDGYRNWL KKAYDVRPEE LMLDRTQLMG 610 620 630 640 650 660 LTAHEMTVLV GGLRVLGTNH NNTQHGVFTD RVGALTNDFF VNLTDMANVW IPSKDNLYEI 670 680 690 700 710 720 RDRKTGNIKW TATRVDLVFG SNSILRSYAE VYAQDDNKEK FVKDFVAAWT KIMNADRFDL A

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed: 15467720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Lens.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR628337 Genomic DNA. Translation: CAH16553.1.
RefSeq	YP_127645.1. NC_006369.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q5WU58.
SMR	Q5WU58. Positions 25-720.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WU58.
Protein family/group databases
PeroxiBase	2644. LpnCP02_Lens.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3115938.
GenomeReviews	Gene locus lpl2313 in contig CR628337_GR.
KEGG	lpf:lpl2313.
NMPDR	fig|297245.3.peg.1667.
PATRIC	22318451. VBILegPne33733_2535.
Organism-specific databases
LegioList	lpl2313.
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q5WU58.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	LPNE297245:LPL2313-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_LEGPL

Accession

Primary (citable) accession number: Q5WU58

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program