Q5WTK7 (FMT_LEGPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	lpl2517

Organism

Legionella pneumophila (strain Lens) [Complete proteome] [HAMAP]

Taxonomic identifier

297245 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella › Legionella pneumophila

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_0000082983
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WTK7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 9E045738412CC9DC	FASTA	314	34,364
	10 20 30 40 50 60 MNGLTVVFAG TPEFGLPCLD ALIQSRHHLK AVYTQPDRPA GRGRKLQESP VKEWAINHQI 70 80 90 100 110 120 SVYQPLNFKN QEAVDELSAL KPDVMVVIAY GLILPKAVLE IPRLGCINVH ASLLPRWRGA 130 140 150 160 170 180 SPIQHAILHG DAESGVTIMQ MDVGLDTGPM LCKAACPVTS SDTAGSLHDK LAKMSVKPLL 190 200 210 220 230 240 DVLEALASNS AQFELQNNEL ATYAGKINKE EARINWHQSA VEIDRKIRAF NPWPVAYTLA 250 260 270 280 290 300 GELMLRIHQA KATDIMSTEM PGMVLNIDKT GMLVATSDNA LLVEKIQFPG AKVISVRDWL 310 NSGKTQLHTG LMLQ

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Lens.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR628337 Genomic DNA. Translation: CAH16757.1.
RefSeq	YP_127846.1. NC_006369.1.
3D structure databases
ProteinModelPortal	Q5WTK7.
ModBase	Search...
Protein-protein interaction databases
STRING	297245.lpl2517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH16757; CAH16757; lpl2517.
GeneID	3115954.
KEGG	lpf:lpl2517.
PATRIC	22318911. VBILegPne33733_2764.
Organism-specific databases
LegioList	lpl2517.
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK833498.
Enzyme and pathway databases
BioCyc	LPNE297245:GJD4-2727-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_LEGPL

Accession

Primary (citable) accession number: Q5WTK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	May 29, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program