Q5WTI9 (DDL_LEGPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Ordered Locus Names:	lpl2535

Organism

Legionella pneumophila (strain Lens) [Complete proteome] [HAMAP]

Taxonomic identifier

297245 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella › Legionella pneumophila

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	D-alanine--D-alanine ligase HAMAP-Rule MF_00047		PRO_1000030460
Regions
	Domain	134 – 347	214	ATP-grasp
	Nucleotide binding	167 – 222	56	ATP By similarity
Sites
	Metal binding	300	1	Magnesium or manganese 1 By similarity
	Metal binding	314	1	Magnesium or manganese 1 By similarity
	Metal binding	314	1	Magnesium or manganese 2 By similarity
	Metal binding	316	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WTI9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DA13815F45D7B9A9	FASTA	364	40,437
	10 20 30 40 50 60 MSKPVNLVLL YGGKSGEHEV SLVSAASVLK HLDSEKYHII PIAMDKSGRF HRHDYNDLLA 70 80 90 100 110 120 CSDKLPVVTE KSTPLEGLLI NGRLAVDAEI VFPVVHGPLY EDGCLQGLLE LAGVAYVGCD 130 140 150 160 170 180 VLSSAIGMDK DMARRLACIN GLKSARYKLL SWHANASERQ QFCHEVASEF GWPLFVKPCS 190 200 210 220 230 240 LGSSVGIHKA NNMDELNAAV ADALRYDEEI LVEEFIVGRE IELAVLENSI PCGKPRVSMV 250 260 270 280 290 300 GEIKVNHPDG YYSYTAKYLE SSQTDLIIPA QLNNSLEEQL KQAAANIFSY LKCKGMARVD 310 320 330 340 350 360 FFVNDKTEEI YFNEINTLPG FTSISMYPKL WQATGIAYPD LLDELINLAM IHHNCRQHLV TNYL

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Lens.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR628337 Genomic DNA. Translation: CAH16775.1.
RefSeq	YP_127864.1. NC_006369.1.
3D structure databases
HSSP	HSSP built from PDB template 1E4E based on UniProtKB P25051.
ProteinModelPortal	Q5WTI9.
ModBase	Search...
Protein-protein interaction databases
STRING	297245.lpl2535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH16775; CAH16775; lpl2535.
GeneID	3116152.
KEGG	lpf:lpl2535.
PATRIC	22318951. VBILegPne33733_2784.
Organism-specific databases
LegioList	lpl2535.
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000011593.
KO	K01921.
OMA	LLHGPFG.
ProtClustDB	PRK01966.
Enzyme and pathway databases
BioCyc	LPNE297245:GJD4-2745-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. False negative. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_LEGPL

Accession

Primary (citable) accession number: Q5WTI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program