Reviewed, UniProtKB/Swiss-Prot Q5WPZ9 (LEU3_BUCCC)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	BCc_PL3

Encoded on

Plasmid pLeu-BCc

Organism

Buchnera aphidicola subsp. Cinara cedri [Complete proteome] [HAMAP]

Taxonomic identifier

372461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	3-isopropylmalate dehydrogenase		PRO_0000274189
Regions
	Nucleotide binding	80 – 93	14	NAD By similarity
	Nucleotide binding	288 – 300	13	NAD By similarity
Sites
	Metal binding	230	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Metal binding	258	1	Magnesium or manganese By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Site	147	1	Important for catalysis By similarity
	Site	198	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WPZ9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: EE0B76D6D4B38690	FASTA	367	41,114
	10 20 30 40 50 60 MKKKKEYRIA VLPGDGIGPE VMQEAYKILN VINKKFDFNI YTNEYDIGGI AIDKYGAPLP 70 80 90 100 110 120 KKTLNGCIES HAILFGSIGG KEWTHLPADE QPERGGLLPL RKFFNLFVNL RPIKLHFSLK 130 140 150 160 170 180 KLSPLKKEII QDGFDILCVR ELIGGIYFGL PKGKNTFDKK NISAFDTEIY STKEIEKIAD 190 200 210 220 230 240 IAFQLSLKRK KKVFSIDKAN VLESSMLWRE VVIQVSKRYP NVELIHLYID NAAMQLIKNP 250 260 270 280 290 300 NQFDVILCSN LFGDILSDEC AALTGSLGML PSASLNESFF GLFEPAGGSA PDLKGKNIAN 310 320 330 340 350 360 PIAQILSLAL LFKYSLQLEK ISHLIELSVL KTLEKGYRTL DISNGKKYIT TDQMGNYIAK TLSELIN

References

[1]	"Plasmids in the aphid endosymbiont Buchnera aphidicola with the smallest genomes. A puzzling evolutionary story." Gil R., Sabater-Munoz B., Perez-Brocal V., Silva F.J., Latorre A. Gene 370:17-25(2006) [PubMed: 16413149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"A small microbial genome: the end of a long symbiotic relationship?" Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A. Science 314:312-313(2006) [PubMed: 17038625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY438025 Genomic DNA. Translation: AAR99734.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus BCc_PL3 in contig AY438025_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BUCCC

Accession

Primary (citable) accession number: Q5WPZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	November 23, 2004
Last modified:	November 4, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)