Q5WNX2 (UPPP2_ENTFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase

Gene names

Name:	uppP
Synonyms:	bcrD

Encoded on

Plasmid pJM01

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin By similarity. HAMAP-Rule MF_01006
Catalytic activity	Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. HAMAP-Rule MF_01006
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01006.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP-Rule MF_01006
Sequence similarities	Belongs to the UppP family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	undecaprenyl-diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Undecaprenyl-diphosphatase HAMAP-Rule MF_01006		PRO_0000151150
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	49 – 69	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Transmembrane	256 – 276	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5WNX2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 70FC7B0C571ED6B7	FASTA	276	30,622
	10 20 30 40 50 60 MALDFIEILK VIFLGIVEGI TEWLPISSTG HMLLVDEFIT LNMSEAFKEM FFVVIQLGAI 70 80 90 100 110 120 LAVVVMFWNK MFPFQFKNKS QSIIKKDTFS LWFKVAVACV PSAIMGILFD DYLDAHLHTP 130 140 150 160 170 180 VVIAIMLILY GVLFIVIENR NKKRTATTST LADISYKTAL MIGVFQVLSL IPGTSRSGAT 190 200 210 220 230 240 IIGALLIGVS RVAAAEFTFF LAVPTMLGAS AFKLLKFGFD FTSAELLTLV IGMAVAFAVS 250 260 270 VFVIKFLMSY IKKHDFKVFG WYRIVLGILV LLITAI

References

[1]

"Acquired bacitracin resistance in Enterococcus faecalis is mediated by an ABC transporter and a novel regulatory protein, BcrR." Manson J.M., Keis S., Smith J.M.B., Cook G.M. Antimicrob. Agents Chemother. 48:3743-3748(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AR01/DGVS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY496968 Genomic DNA. Translation: AAS78449.1.
RefSeq	YP_004032990.1. NC_014726.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9988300.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase.
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP2_ENTFL

Accession

Primary (citable) accession number: Q5WNX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program