Reviewed, UniProtKB/Swiss-Prot Q5WLV8 (ISPE_BACSK)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	ABC0074

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235063
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WLV8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E1B030AE92A52018	FASTA	297	32,110
	10 20 30 40 50 60 MKYSIKAPAK INLSLDVIRK REDGYHEVEM IMTMVDLADR VDLSLRADGG ISIDVSEGFV 70 80 90 100 110 120 PSDERNFAYQ AASLLKKRYN VREGVHIYIT KRIPVAAGLA GGSSDAAATL KGLNELWQLG 130 140 150 160 170 180 LSVDELATLG AEIGSDVSFC VYGGTALATG RGELIKPIAS PPPLWVILAK PPIGLSTADV 190 200 210 220 230 240 YNGLKLSEAR HAKTAEMIAA LERQDAASIC ALLHNTLEDV TLRLYPEVAH IKEQMKRFGA 250 260 270 280 290 NGVLMSGSGP TVFGIVEKES RVNRIYNGLR GFCDEVYAVR LIRTSGTCPN TDENGML

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD62617.1.
RefSeq	YP_173578.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WLV8.
Genome annotation databases
GeneID	3204037.
GenomeReviews	Gene locus ABC0074 in contig AP006627_GR.
KEGG	bcl:ABC0074.
NMPDR	fig|66692.3.peg.1040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WLV8.
OMA	DGYHEVE.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC0074-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BACSK

Accession

Primary (citable) accession number: Q5WLV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)