Reviewed, UniProtKB/Swiss-Prot Q5WLE9 (GLUQ_BACSK)

Names and origin

Protein names

Recommended name:
    Glutamyl-Q tRNA(Asp) synthetase
      Short name=Glu-Q-RSs
    EC=6.1.1.-

Gene names

Name:	gluQ
Ordered Locus Names:	ABC0263

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428		PRO_0000208286
Regions
	Region	7 – 11	5	Glutamate binding By similarity
	Motif	10 – 20	11	"HIGH" region HAMAP MF_01428
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_01428
Sites
	Metal binding	107	1	Zinc By similarity
	Metal binding	109	1	Zinc By similarity
	Metal binding	131	1	Zinc By similarity
	Metal binding	135	1	Zinc By similarity
	Binding site	43	1	Glutamate By similarity
	Binding site	192	1	Glutamate By similarity
	Binding site	210	1	Glutamate By similarity
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WLE9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: BA1EB1BDB1A19FBB	FASTA	318	35,381
	10 20 30 40 50 60 MNQLRGRFAP TPSGELHLGN LYVALLSWLQ VRQGGGQFIL RMEDIDRPRS RNELVGPMMD 70 80 90 100 110 120 DLHWLGLDWD EGPDQGGENG PYYQSKRLHL YEEALHNLQQ QQLLYHCYCS RAELQAVASA 130 140 150 160 170 180 PHGVASEGQA YPGTCKKLTA AERAAKAAKK TPSLRFAVDK GPTAEKMDVE MEKLLRRGGD 190 200 210 220 230 240 FVVQRADGII SYQLAVVVDD ALMGITDVLR GQDLYDSTPR QLLLYAALGY TAPRFVHVPL 250 260 270 280 290 300 LYGPDGRRLA KRHRDLSLAA IRANGCRPEN IVGLLAALSG LIERPEPLTA AELIPEFRLE 310 KLTTEPILLD THLLQLLN

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD62806.1.
RefSeq	YP_173767.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WLE9.
Genome annotation databases
GeneID	3202275.
GenomeReviews	Gene locus ABC0263 in contig AP006627_GR.
KEGG	bcl:ABC0263.
NMPDR	fig|66692.3.peg.1359.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WLE9.
OMA	QPVRRQS.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC0263-MON.
Family and domain databases
HAMAP	MF_01428. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLUQ_BACSK

Accession

Primary (citable) accession number: Q5WLE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)