Reviewed, UniProtKB/Swiss-Prot Q5WL08 (G1PDH_BACSK)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Synonyms:	araM
Ordered Locus Names:	ABC0405

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350639
Regions
	Nucleotide binding	114 – 118	5	NAD By similarity
	Nucleotide binding	136 – 139	4	NAD By similarity
Sites
	Metal binding	188	1	Nickel; catalytic By similarity
	Metal binding	268	1	Nickel; catalytic By similarity
	Metal binding	288	1	Nickel; catalytic By similarity
	Binding site	52	1	NAD By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	145	1	NAD By similarity
	Binding site	188	1	Substrate By similarity
	Binding site	272	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WL08-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 0498F7DC2474BB69	FASTA	394	42,745
	10 20 30 40 50 60 MNISALKARI RASKEDLYPL PLEQIVVAEG ALREAAAFIA KQAESGVVLV ADSHTYKAAG 70 80 90 100 110 120 RDLHDHLCQK GVKADWHLLE PNSNGDIIAD EVSLISVMLK VEQSTKLLVA VGAGTIHDIV 130 140 150 160 170 180 RFVSGKTNKP FVSIPTAPSV DGFTSLGAPI VVQGSKKTYQ LVAPIALFAD TVVLQHAPPA 190 200 210 220 230 240 LIAAGFGDMV GKYTSLFDWK IGSLLKNEAY SDLVADLTEE ALSTCVDKVA DIGKRTSAGV 250 260 270 280 290 300 QVLMESLLTS GLAMALFGYS HPASGAEHHL SHYWEMEALR LGKKQLLHGA KVGLSTQIIN 310 320 330 340 350 360 RFYKNTVLPD VHRFLDDDEA KQVIALVEAL PDPDDVKALL EQAGWNEALV PIAPELVEES 370 380 390 LQHAYLLRDR YTLLRLYREG TTMKKEGDAH VGRS

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD62947.1.
RefSeq	YP_173908.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WL08.
Genome annotation databases
GeneID	3201709.
GenomeReviews	Gene locus ABC0405 in contig AP006627_GR.
KEGG	bcl:ABC0405.
NMPDR	fig|66692.3.peg.217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WL08.
OMA	LHGAKVG.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G1PDH_BACSK

Accession

Primary (citable) accession number: Q5WL08

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)