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Q5WKZ1 (IOLA1_BACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylmalonate semialdehyde dehydrogenase [acylating] 1
Short name=MMSA dehydrogenase 1
Short name=MMSDH 1
Short name=MSDH 1
EC=1.2.1.27
Alternative name(s):
Malonate semialdehyde dehydrogenase [acetylating] 1
Short name=MSA dehydrogenase 1
EC=1.2.1.18
Gene names
Name:iolA1
Ordered Locus Names:ABC0422
OrganismBacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]
Taxonomic identifier66692 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity. HAMAP MF_01670

Catalytic activity

3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670

Pathway

Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670

Subunit structure

Homotetramer By similarity. HAMAP MF_01670

Sequence similarities

Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Methylmalonate semialdehyde dehydrogenase [acylating] 1 HAMAP MF_01670
PRO_0000352326

Regions

Nucleotide binding180 – 1845NAD By similarity

Sites

Active site2881Nucleophile By similarity
Binding site3861NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5WKZ1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: D12D2FFD4698A92B

FASTA49153,477
        10         20         30         40         50         60 
MGETTVEVRK VKNYINGKWI ESSTSLYEDV VNPATKEVIC QVPLSTKADV EYAVEAAKTA 

        70         80         90        100        110        120 
FAKWSKVAVP RRARILFNYQ QLLQRDKEEL ARLITIENGK NLTEARGEVQ RGIENVEFAA 

       130        140        150        160        170        180 
GAPSLMMGDS LPMIATDVEA SNYRYPIGVV GGIAPFNFPM MVPCWMFPMA IALGNAFLLK 

       190        200        210        220        230        240 
PSERTPLLTE KLADLLTEAG VPDGVFNVVY GAHDVVNGML EHPDIKAISF VGSKPVGEYV 

       250        260        270        280        290        300 
YKKASENLKR VQALTGAKNH TIVLNDADLD DAVTNIAGAA FGSAGERCMA CAVVTVEEGI 

       310        320        330        340        350        360 
ADAFVAKLKA KAESLVMGNG LDDGVFLGPV IREENKNRTI AYIEKGVEEG ATLVCDGRSR 

       370        380        390        400        410        420 
VSADGYFIGP TIFENVTTDM TIWKDEIFAP VLSIIRVKNL DEGIQIANRS EFANGACLFT 

       430        440        450        460        470        480 
TNAAAVRYFR ENIDAGMLGI NLGVPAPMAF FPFSGWKSSF YGTLHANGKD SVDFYTRKKV 

       490 
VTARYPKPSF D

« Hide

References

[1]"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KSM-K16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006627 Genomic DNA. Translation: BAD62964.1.
RefSeqYP_173925.1. NC_006582.1.

3D structure databases

HSSPHSSP built from PDB template 1T90 based on UniProtKB P42412.
ProteinModelPortalQ5WKZ1.
SMRQ5WKZ1. Positions 7-490.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5WKZ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000047184; EBBACP00000045945; EBBACG00000047175.
GeneID3201317.
GenomeReviewsGene locus ABC0422 in contig AP006627_GR.
KEGGbcl:ABC0422.
NMPDRfig|66692.3.peg.325.
PATRIC18920072. VBIBacCla58185_0441.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
GeneTreeEBGT00070000031871.
HOGENOMHBG752218.
OMAYAEGTRR.
ProtClustDBCLSK2460970.

Enzyme and pathway databases

BioCycBCLA66692:ABC0422-MONOMER.

Family and domain databases

HAMAPMF_01670. IolA.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
IPR023510. MeMal-semiAld_DH_GmP_bac.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KOK00140.
PANTHERPTHR11699:SF27. MMSDH. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR01722. MMSDH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIOLA1_BACSK
AccessionPrimary (citable) accession number: Q5WKZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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