Reviewed, UniProtKB/Swiss-Prot Q5WKZ1 (IOLA1_BACSK)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 1
      Short name=MMSA dehydrogenase 1
      Short name=MMSDH 1
      Short name=MSDH 1
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 1
      Short name=MSA dehydrogenase 1
    EC=1.2.1.18

Gene names

Name:	iolA1
Ordered Locus Names:	ABC0422

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Methylmalonate semialdehyde dehydrogenase [acylating] 1 HAMAP MF_01670		PRO_0000352326
Regions
	Nucleotide binding	180 – 184	5	NAD By similarity
Sites
	Active site	288	1	Nucleophile By similarity
	Binding site	386	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WKZ1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D12D2FFD4698A92B	FASTA	491	53,477
	10 20 30 40 50 60 MGETTVEVRK VKNYINGKWI ESSTSLYEDV VNPATKEVIC QVPLSTKADV EYAVEAAKTA 70 80 90 100 110 120 FAKWSKVAVP RRARILFNYQ QLLQRDKEEL ARLITIENGK NLTEARGEVQ RGIENVEFAA 130 140 150 160 170 180 GAPSLMMGDS LPMIATDVEA SNYRYPIGVV GGIAPFNFPM MVPCWMFPMA IALGNAFLLK 190 200 210 220 230 240 PSERTPLLTE KLADLLTEAG VPDGVFNVVY GAHDVVNGML EHPDIKAISF VGSKPVGEYV 250 260 270 280 290 300 YKKASENLKR VQALTGAKNH TIVLNDADLD DAVTNIAGAA FGSAGERCMA CAVVTVEEGI 310 320 330 340 350 360 ADAFVAKLKA KAESLVMGNG LDDGVFLGPV IREENKNRTI AYIEKGVEEG ATLVCDGRSR 370 380 390 400 410 420 VSADGYFIGP TIFENVTTDM TIWKDEIFAP VLSIIRVKNL DEGIQIANRS EFANGACLFT 430 440 450 460 470 480 TNAAAVRYFR ENIDAGMLGI NLGVPAPMAF FPFSGWKSSF YGTLHANGKD SVDFYTRKKV 490 VTARYPKPSF D

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD62964.1.
RefSeq	YP_173925.1.
3D structure databases
SMR	Q5WKZ1. Positions 7-490.
ModBase	Search...
Genome annotation databases
GeneID	3201317.
GenomeReviews	Gene locus ABC0422 in contig AP006627_GR.
KEGG	bcl:ABC0422.
NMPDR	fig|66692.3.peg.325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WKZ1.
OMA	Q5WKZ1. LINQHEF.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA1_BACSK

Accession

Primary (citable) accession number: Q5WKZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)