Q5WKY8 (IOLD_BACSK) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 47.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase Short name=THcHDO hydrolase EC=3.7.1.n2 | ||||
| Gene names |
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| Organism | Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 66692 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 637 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity. HAMAP MF_01669 |
| Catalytic activity | 3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01669 Binds 1 thiamine pyrophosphate per subunit By similarity. |
| Pathway | Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669 |
| Sequence similarities | Belongs to the TPP enzyme family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Magnesium Metal-binding NAD Thiamine pyrophosphate |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | inositol catabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: InterPro thiamine pyrophosphate bindingInferred from electronic annotation. Source: InterPro transferase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 637 | 637 | 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669 | PRO_0000352532 | |||||
Regions | |||||||||
| Region | 442 – 522 | 81 | Thiamine pyrophosphate binding By similarity | ||||||
Sites | |||||||||
| Metal binding | 493 | 1 | Magnesium By similarity | ||||||
| Metal binding | 520 | 1 | Magnesium By similarity | ||||||
| Binding site | 66 | 1 | Thiamine pyrophosphate By similarity | ||||||
Sequences
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References
| [1] | "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AP006627 Genomic DNA. Translation: BAD62967.1. |
| RefSeq | YP_173928.1. NC_006582.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1JSC based on UniProtKB P07342. |
| ProteinModelPortal | Q5WKY8. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5WKY8. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000046167; EBBACP00000044928; EBBACG00000046158. |
| GeneID | 3201320. |
| GenomeReviews | Gene locus ABC0425 in contig AP006627_GR. |
| KEGG | bcl:ABC0425. |
| NMPDR | fig|66692.3.peg.328. |
| PATRIC | 18920078. VBIBacCla58185_0444. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG3962. |
| GeneTree | EBGT00070000032034. |
| HOGENOM | HBG294332. |
| OMA | IYAVTSS. |
| ProtClustDB | CLSK2300076. |
Enzyme and pathway databases | |
| BioCyc | BCLA66692:ABC0425-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01669. IolD. [Tree] |
| InterPro | IPR023757. THcHDO_hydrolase. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view] |
| KO | K03336. |
| Pfam | PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view] |
| PROSITE | PS00187. TPP_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IOLD_BACSK | ||||||||
| Accession | Primary (citable) accession number: Q5WKY8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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