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Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • sirohemeUniRule annotationNote: Binds 1 siroheme per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] hemoprotein beta-component (cysI), Sulfite reductase [NADPH] flavoprotein alpha-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi438 – 4381Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi444 – 4441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi483 – 4831Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi487 – 4871Iron (siroheme axial ligand)UniRule annotation
Metal bindingi487 – 4871Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-649-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-HPUniRule annotation
Short name:
SiRHPUniRule annotation
Gene namesi
Name:cysIUniRule annotation
Ordered Locus Names:ABC0619
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573Sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000388474Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC0619.

Structurei

3D structure databases

ProteinModelPortaliQ5WKE7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QHM. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
KOiK00381.
OMAiGKYNMYL.
OrthoDBiPOG091H04FQ.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI. 1 hit.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5WKE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQKFAPTQ DGPPSDVERI KEESNYLRGT LAESLNEPLS AGIPDDDNRL
60 70 80 90 100
MKFHGSYLQD DRDLREERRR QKLEPAYQFM VRVRLPGGVA TPKQWLAMDD
110 120 130 140 150
LAHRYGNGTL RLTTRQTFQM HGILKWNMKK NIQGINAALM DTIAACGDVN
160 170 180 190 200
RNVMSTPNPY QSEVHGEVYD WAKKLSEALL PKTRAYHEIW LDEEKVAGTP
210 220 230 240 250
QQDEVEPMYG PLYLPRKFKI GVAVPPANDV DIFSQDIGFI AILEEGKLTG
260 270 280 290 300
FNVACGGGMG MTHGDTKTYP QLGRIIGFCE PQQIMDVAEK LITIQRDYGN
310 320 330 340 350
RSVRKYARFK YTIDRRGLDW LKNELHARLG WELGEARPFQ FDHNGDRYGW
360 370 380 390 400
TKGVKGRWHL TLFIQNGRIQ DTDDYQLMTG LREIAKIHTG EFRLTGNQNV
410 420 430 440 450
IISNITAAKK KAIQAIVETY RLTDGQHVSA LRRNSMACVA FPTCGLAMAE
460 470 480 490 500
SERYLPSLID KLEFILDEAG IREEDIVIRM AGCPNGCSRA ALAEIGFIGK
510 520 530 540 550
APGKYNLYLG GGFAGERLSK MYKENIGEAE ILETLEPILF AYAKEREEGE
560 570
HFGDFVIRKG YVKAVESGTD FHQ
Length:573
Mass (Da):64,743
Last modified:November 23, 2004 - v1
Checksum:i38EFAB88740A4063
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63158.1.
RefSeqiWP_011245474.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD63158; BAD63158; ABC0619.
KEGGibcl:ABC0619.
PATRICi18920490. VBIBacCla58185_0650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63158.1.
RefSeqiWP_011245474.1. NC_006582.1.

3D structure databases

ProteinModelPortaliQ5WKE7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi66692.ABC0619.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD63158; BAD63158; ABC0619.
KEGGibcl:ABC0619.
PATRICi18920490. VBIBacCla58185_0650.

Phylogenomic databases

eggNOGiENOG4107QHM. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
KOiK00381.
OMAiGKYNMYL.
OrthoDBiPOG091H04FQ.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciBCLA66692:GHMP-649-MONOMER.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI. 1 hit.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSI_BACSK
AccessioniPrimary (citable) accession number: Q5WKE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.