Reviewed, UniProtKB/Swiss-Prot Q5WJS9 (ARSC_BACSK)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:	arsC
Ordered Locus Names:	ABC0837

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity. HAMAP MF_01624
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity. HAMAP MF_01624
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 139	139	Protein arsC HAMAP MF_01624		PRO_0000162516
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	82	1	Nucleophile; for reductase activity By similarity
	Active site	89	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 82		Redox-active; alternate By similarity
	Disulfide bond	82 ↔ 89		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WJS9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1CEED8D2B9BE64F9	FASTA	139	15,567
	10 20 30 40 50 60 MSKKIIYFLC TGNSCRSQMA EGFGKHYLSD EWEVYSAGME AHGLNPNAVK AMNEVGIDIS 70 80 90 100 110 120 DQTSDLIHID LLNNADFVVT LCGDAADKCP LTPAHVKREH WGFDDPAKAE GTKEERWMVF 130 QRVRDEIGAR IKKFAETGE

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD63376.1.
RefSeq	YP_174337.1.
3D structure databases
SMR	Q5WJS9. Positions 1-139.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WJS9.
Genome annotation databases
GeneID	3201730.
GenomeReviews	Gene locus ABC0837 in contig AP006627_GR.
KEGG	bcl:ABC0837.
NMPDR	fig|66692.3.peg.89.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0394.
HOGENOM	HBG730979.
OMA	WHKSKAT.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC0837-MONOMER.
Family and domain databases
HAMAP	MF_01624. Arsenate_reduct. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_BACSK

Accession

Primary (citable) accession number: Q5WJS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)