Q5WJ87 (PURQ_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	ABC1029

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus clausii

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Phosphoribosylformylglycinamidine synthase 1 HAMAP-Rule MF_00421		PRO_0000100538
Regions
	Domain	3 – 227	225	Glutamine amidotransferase type-1
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	194	1	By similarity
	Active site	196	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WJ87 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 8C3BABCB7A0A930E	FASTA	227	24,776
	10 20 30 40 50 60 MKFAVIVFPG SNCDADMYHA IKDGLGEEVE YVFHTETSLD GFDAVLLPGG FSHGDYLRSG 70 80 90 100 110 120 AIARFAPIMP AVIQAAEAGK PVLGVCNGFQ VLLEAGLLPG AMKRNIDLKF VCRTVELVVE 130 140 150 160 170 180 NNETMFSSGY EQGQTIRIPV AHGEGNYECD DETLAKLREN KQIVFRYNEH VNGSKGDIAG 190 200 210 220 ITNERGNVLG MMPHPERATE TLLGNDQGLA VFTSILRNWR ESHVTAS

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD63568.1.
RefSeq	YP_174529.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WJ87.
ModBase	Search...
Protein-protein interaction databases
STRING	66692.ABC1029.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD63568; BAD63568; ABC1029.
GeneID	3202816.
KEGG	bcl:ABC1029.
PATRIC	18921424. VBIBacCla58185_1100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0047.
HOGENOM	HOG000238240.
KO	K01952.
OMA	FPGTNCD.
ProtClustDB	PRK03619.
Enzyme and pathway databases
BioCyc	BCLA66692:GHMP-1026-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00421. PurQ.
InterPro	IPR017926. GATASE_1. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_BACSK

Accession

Primary (citable) accession number: Q5WJ87

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program