Reviewed, UniProtKB/Swiss-Prot Q5WHT9 (SERC_BACSK)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	ABC1531

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Phosphoserine aminotransferase HAMAP MF_00160		PRO_0000150152
Regions
	Region	76 – 77	2	Pyridoxal phosphate binding By similarity
	Region	234 – 235	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	42	1	L-glutamate By similarity
	Binding site	100	1	Pyridoxal phosphate By similarity
	Binding site	150	1	Pyridoxal phosphate By similarity
	Binding site	169	1	Pyridoxal phosphate By similarity
	Binding site	192	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	193	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WHT9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 63912AEA2BEC72C6	FASTA	364	40,021
	10 20 30 40 50 60 MNDVHNFNAG PAALPKQALE RAQKELVNFD GTGMSVMELS HRGDTYDRIH THAIKKIRKL 70 80 90 100 110 120 LDVPDDYHIL FLQGGASLQF PMVPMNFLDS QATYILTGAW SEKALAEAKH FGKTVIGASG 130 140 150 160 170 180 KDGNYKQIPA LNQISEHAQD SYVHMTSNNT IYGTQWHAFP NTKAPLVCDM SSDIFSRRIP 190 200 210 220 230 240 VNQFGLIYAG AQKNLGPSGV TLVLIQDAFL QRAKSGLPAM LSYDTFVKSN SLYNTPPVFS 250 260 270 280 290 300 IYMLGLVLDW LEESGGLTEI ENRNKAKAQL LYEAIDESDG FYIGHAAQDS RSNMNVTFTL 310 320 330 340 350 360 ANDALTSVFL QEAKEAGFVG LNGHRSVGGL RASIYNAVPY ASCEALVDFM KQFKNKHAEK GATV

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64066.1.
RefSeq	YP_175027.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WHT9.
Genome annotation databases
GeneID	3200684.
GenomeReviews	Gene locus ABC1531 in contig AP006627_GR.
KEGG	bcl:ABC1531.
NMPDR	fig|66692.3.peg.1905.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WHT9.
OMA	SMYNTPP.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1531-MON.
Family and domain databases
HAMAP	MF_00160. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_BACSK

Accession

Primary (citable) accession number: Q5WHT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)