Reviewed, UniProtKB/Swiss-Prot Q5WHT1 (HEMH_BACSK)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	ABC1539

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Ferrochelatase HAMAP MF_00323		PRO_0000175113
Sites
	Metal binding	181	1	Iron By similarity
	Metal binding	262	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WHT1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1DB40513077CC107	FASTA	312	35,495
	10 20 30 40 50 60 MEKKKIGLLV MAYGTPRKKE EIEPYYTHIR HGRKPSEEAL ADLQERYEAI GGTSPLARIT 70 80 90 100 110 120 DEQANGLAAV LNEQQEDIVF VPYLGLKHID PFIEDAVQKM KNDGIETAVS IVLAPHFSTF 130 140 150 160 170 180 SVQSYNKRAQ EEADRIGGPT IYTIESWYKE PGFLQFWKEQ IENEMKKADS IDDLCVIFSA 190 200 210 220 230 240 HSLPEKIIQA NDPYPEQLRE TADLLADMAN VPNYEIGWQS EGNTPEPWLG PDVQDLTREL 250 260 270 280 290 300 YAKKGYKTFM YCPVGFVAEH LEVLYDNDYE CKLVTDELGV RYLRPPMPNA DTRFLKALAD 310 VVTKRLAAVV TK

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64074.1.
RefSeq	YP_175035.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WHT1.
Genome annotation databases
GeneID	3204570.
GenomeReviews	Gene locus ABC1539 in contig AP006627_GR.
KEGG	bcl:ABC1539.
NMPDR	fig|66692.3.peg.1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WHT1.
OMA	VKWLEPS.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1539-MON.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BACSK

Accession

Primary (citable) accession number: Q5WHT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)