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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Proton acceptorUniRule annotation1
Binding sitei149SubstrateUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Binding sitei222Substrate; via amide nitrogenUniRule annotation1
Active sitei272NucleophileUniRule annotation1
Metal bindingi310ZincUniRule annotation1
Metal bindingi312ZincUniRule annotation1
Metal bindingi315ZincUniRule annotation1
Metal bindingi341Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processQueuosine biosynthesis, tRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBCLA66692:G1G25-1661-MONOMER
UniPathwayiUPA00392

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:ABC1558
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001354511 – 383Queuine tRNA-ribosyltransferaseAdd BLAST383

Proteomic databases

PRIDEiQ5WHR2

Interactioni

Subunit structurei

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC1558

Structurei

3D structure databases

ProteinModelPortaliQ5WHR2
SMRiQ5WHR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 99Substrate bindingUniRule annotation5
Regioni253 – 259RNA bindingUniRule annotation7
Regioni277 – 281RNA binding; important for wobble base 34 recognitionUniRule annotation5

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U Bacteria
COG0343 LUCA
HOGENOMiHOG000223473
KOiK00773
OMAiTYHLFLR
OrthoDBiPOG091H00XO

Family and domain databases

Gene3Di3.20.20.105, 1 hit
HAMAPiMF_00168 Q_tRNA_Tgt, 1 hit
InterProiView protein in InterPro
IPR004803 TGT
IPR036511 TGT-like_sf
IPR002616 tRNA_ribo_trans-like
PfamiView protein in Pfam
PF01702 TGT, 1 hit
SUPFAMiSSF51713 SSF51713, 1 hit
TIGRFAMsiTIGR00430 Q_tRNA_tgt, 1 hit
TIGR00449 tgt_general, 1 hit

Sequencei

Sequence statusi: Complete.

Q5WHR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAITYEHI KTCKQSGARL GRVHTPHGTI ETPMFMPVGT LATVKTMSPE
60 70 80 90 100
DLKAMDAQII LSNTYHLWLR PGEEIIKEAG GLHQFMNWDK PILTDSGGFQ
110 120 130 140 150
VFSLSDLRKI TEEGVHFRNH LSGEKLFLSP EKAMQIQHVL GSDIMMAFDE
160 170 180 190 200
CPPYPADHSY MKASVERTSR WAERCLAEHK RSGKDSVQGL FGIIQGGEYE
210 220 230 240 250
DLRKQSANDL TALDFPGYAV GGLSVGEPKD VMNHVLEFTT PLMPEDKPRY
260 270 280 290 300
LMGVGSPDSL IDGAIRGIDM FDCVLPTRIA RNGTCMTSAG RLVVRNAKYA
310 320 330 340 350
RDFSPLDEKC DCHVCRTYSR AYIRHLIKCE ETFGFRLTTY HNLYFLLNLM
360 370 380
KQVRQAIMDD CLLDFREQFF EEYGFNKENA RNF
Length:383
Mass (Da):43,432
Last modified:November 23, 2004 - v1
Checksum:i68FFD9AA8D4FC6C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA Translation: BAD64093.1
RefSeqiWP_011246402.1, NC_006582.1

Genome annotation databases

EnsemblBacteriaiBAD64093; BAD64093; ABC1558
GeneIDi34044458
KEGGibcl:ABC1558

Similar proteinsi

Entry informationi

Entry nameiTGT_BACSK
AccessioniPrimary (citable) accession number: Q5WHR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health