Reviewed, UniProtKB/Swiss-Prot Q5WHP3 (SYD_BACSK)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	ABC1577

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00044
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110828

Sequences

	Sequence		Length	Mass (Da)
	Q5WHP3-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: BF9C8E7E4AB71F4C	FASTA	589	66,682
	10 20 30 40 50 60 MKRTHHCGQL DKTLAGQQVQ LKGWVQRRRD LGQVIFLDLR DRSGIVQVVC SPEVSEEALK 70 80 90 100 110 120 AADRVRNEYL VAVSGIVVER NEQAVNDKLA TGAIEVHVRE LEILNVSKSL PFQIEADTDA 130 140 150 160 170 180 AEDVRLKYRY LDLRRPDMQE AFAMRHKIMK SVRDFLDADG YLEIETPMLT KSTPEGARDY 190 200 210 220 230 240 LVPSRVHHGQ FYALPQSPQI FKQLLMVSGF EKYFQIVRCF RDEDLRADRQ PEFTQIDIET 250 260 270 280 290 300 SFLDTEDILA MTEAMMEKLL KETHGLELKR PFSRMTYEEA MNRYGSDKPD TRFGMELIEL 310 320 330 340 350 360 SDVLKDTEFK VFKGALEAGG IIKGLTLKGG ADKLSRKDID ALADFVKPYG AKGLAWLKVD 370 380 390 400 410 420 DEGIKGPIAK FFNEAQTAAL LAAMDAEAGD LLFFGADKKQ VVFHALGALR LKFGKEFQLI 430 440 450 460 470 480 DENAFHFLWV TDFPLVEYDE QAKRFVALHH PFTRPKAEDL DLMETHPEQV RAEAYDLVLN 490 500 510 520 530 540 GFELGGGSQR IYERELQEKM FKLLGFSSEA AKEEFGFLLE AFDYGTPPHG GIALGLDRIV 550 560 570 580 MLLARKSNLR EVIAFPKTAS ASDLLTEAPN KVSVEQLIDL NLSIISKRS

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64112.1.
RefSeq	YP_175073.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WHP3.
Genome annotation databases
GeneID	3201736.
GenomeReviews	Gene locus ABC1577 in contig AP006627_GR.
KEGG	bcl:ABC1577.
NMPDR	fig|66692.3.peg.1539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WHP3.
OMA	VDRRRDH.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1577-MON.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACSK

Accession

Primary (citable) accession number: Q5WHP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)