Reviewed, UniProtKB/Swiss-Prot Q5WHM8 (SYA_BACSK)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ABC1592

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075054

Sequences

	Sequence		Length	Mass (Da)
	Q5WHM8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 5AB72CAF3457DC56	FASTA	876	97,328
	10 20 30 40 50 60 MQRLSSASVR QMFIDFFKEK GHSVEPSASL VPFEDPSLLW INSGVATLKK YFDGRVIPEN 70 80 90 100 110 120 PRIVNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEQAIEWAWE FLTDKKWIGF 130 140 150 160 170 180 PQERLSVTVH PEDEEAYRYW HQHIGLPEER IIRLEGNFWD IGEGPSGPNS EIFYDRGPEY 190 200 210 220 230 240 GDDPNDPELY PGGENERYLE IWNLVFSQFN HNADGTYTPL PKKNIDTGMG LERMVSVIQD 250 260 270 280 290 300 TPTNFETDLF MPIIEATEAL ASTTYKEADT AFKVIADHIR TVAFAVGDGA LPSNEGRGYV 310 320 330 340 350 360 LRRLLRRAVR FAKSIGIDRP FMYELVPVVG AIMKDFYPEV ADKQDFIARV IRTEEERFHE 370 380 390 400 410 420 TLNEGLAILS DIIEKAKATN KETIAGEDAF RLYDTYGFPI DLTEEYVHDE GLTVDRAGFD 430 440 450 460 470 480 QEMEQQRQRA RAARQESASM SVQEDVFGEV KTPSVFVGYE HTETDAVITT LVRGKEKVEQ 490 500 510 520 530 540 AVQGDVIQFF LDETPFYAES GGQVADRGMI VTDTGQAVVK DVKKAPNGQH LHTAEVTIGE 550 560 570 580 590 600 ISTGQQASAR IEVRERLDIV KNHTATHLLH QALKDVLGEH VNQAGSLVSS ERLRFDFSHF 610 620 630 640 650 660 GQVTPNELQQ IEEIVNEKVW QALPVDISIQ RLEEAKAAGA MALFGEKYGS EVRVVRVGDY 670 680 690 700 710 720 SLELCGGCHV RNTAEIGLFK ITSESGIGAG VRRIEAVTSK GAYQFLSEQT AILKDAAERL 730 740 750 760 770 780 KAKRLSDVPQ RIESLQEELR KAQRENESLT AKLGQAEAGN LNDQVKEIGG VAVIAAQVDA 790 800 810 820 830 840 KDTEALRSMV DTLKQAHEKA VIVLAAKTGN KLAFVAGVTK PAIAEGFHAG KLIKEVAART 850 860 870 GGGGGGRPDM AQAGGKDPAK LDEALAYVHE YVKSIS

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64127.1.
RefSeq	YP_175088.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WHM8.
Genome annotation databases
GeneID	3201695.
GenomeReviews	Gene locus ABC1592 in contig AP006627_GR.
KEGG	bcl:ABC1592.
NMPDR	fig|66692.3.peg.1554.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WHM8.
OMA	ASGKHND.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1592-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACSK

Accession

Primary (citable) accession number: Q5WHM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)