Q5WHC6 (ISPH_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	ABC1694

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128775

Sequences

	Sequence		Length	Mass (Da)
	Q5WHC6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 982266D4D36DFA86	FASTA	314	34,882
	10 20 30 40 50 60 MDVLKISPRG YCYGVVDAMV MARQAAQNLD LPRPLYILGQ IVHNQHVTDA FKEDGIISLD 70 80 90 100 110 120 GPNRLEILKN VTHGTVIFTA HGVSPEVRRL AKEKGLTCID ATCPDVTRTH DLIREKAEEG 130 140 150 160 170 180 YQFIYIGKQG HPEPEGAMGV APDSVHLVET MEDLEQLTLD DRDIIITNQT TMSQWDVADI 190 200 210 220 230 240 MKRAMELYPN AEVHNEICLA TQVRQEAVAE QASQCDIVIV VGDPKSNNSN RLAQVSEQIA 250 260 270 280 290 300 GTPAYRIGDL SELKLEWIKE AKKVGVTSGA STPTPITKEV ITFIENYDPN DESTWDTAKK 310 VPIEKILPKV RVKK

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64229.1.
RefSeq	YP_175190.1. NC_006582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WHC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000046680; EBBACP00000045441; EBBACG00000046671.
GeneID	3201112.
GenomeReviews	Gene locus ABC1694 in contig AP006627_GR.
KEGG	bcl:ABC1694.
NMPDR	fig|66692.3.peg.1635.
PATRIC	18922864. VBIBacCla58185_1800.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
GeneTree	EBGT00050000002965.
HOGENOM	HBG335228.
OMA	HNQGKRY.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1694-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_BACSK

Accession

Primary (citable) accession number: Q5WHC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program