Reviewed, UniProtKB/Swiss-Prot Q5WH54 (PROA_BACSK)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	ABC1766

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase		PRO_0000189695

Sequences

	Sequence		Length	Mass (Da)
	Q5WH54-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: A15EECC7BAB0DCA7	FASTA	415	44,511
	10 20 30 40 50 60 MATAKLISQK AKAHAPSLSQ ATTAEKNSVL QAMAQQLKAD ADVLLQANQL DIANAKKNGL 70 80 90 100 110 120 SDHLIDRLLL TKARIDAMAT AIDHLIELPE PIGAVKEEIK RPNGLVIKEM VVPFGVVGMI 130 140 150 160 170 180 YEARPNVTVD ACTLAVKTGN AVVLRGSASA LHSNKAIVHT LKKAFQNSPI HPDVIQLLED 190 200 210 220 230 240 TSKEEAANMM QLKSTIDVLI PRGGANLIQT VLQEATIPVI ETGVGNCHVY VASSADPDMA 250 260 270 280 290 300 FDIVVNAKTQ RPSVCNACET LLVEQSFAKA HLPALVERLS AKGVSLVGNE GACTLDPRIA 310 320 330 340 350 360 QANEDDWSTE YLDLRLAIRV VADTNEAIAH INQYGTKHSE AIITESLAER DTFFATVDAA 370 380 390 400 410 CVYHNASTRF TDGFEFGFGA EIGISTQKLH ARGPMGLKAL TTSKYGIYGE GQVKE

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64301.1.
RefSeq	YP_175262.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3202777.
GenomeReviews	Gene locus ABC1766 in contig AP006627_GR.
KEGG	bcl:ABC1766.
NMPDR	fig|66692.3.peg.1714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WH54.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1766-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BACSK

Accession

Primary (citable) accession number: Q5WH54

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)