Reviewed, UniProtKB/Swiss-Prot Q5WH53 (PROB_BACSK)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	ABC1767

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109640
Regions
	Domain	276 – 353	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q5WH53-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 8B2175BAB7678BCA	FASTA	372	39,869
	10 20 30 40 50 60 MATGTIVIKI GSSSLTDGNG ALSTERLRGH VEAICELRHQ GINVIVVTSG AVAAGFTLIG 70 80 90 100 110 120 YHQRPKSISQ KQAAAAVGQS LLMQAYMDAF RLHGYTAAQL LLTRADFADP ARFSNISQTL 130 140 150 160 170 180 RELLKRGAIP VINENDSTSV DELTFGDNDR LSALVSGIIH ADMLCLYTDV NGVYDQNPND 190 200 210 220 230 240 SPNAKKYHYL ASVPEALLDR VSADTSAVGT GGMKSKLLAA KTAIELGTNV FIGNGKGRET 250 260 270 280 290 300 FTDVLLGKGD GTYLGPFTNK TMSTTKQWIV FHSEPAGSIE IDSGASTALL KRGKSLLPAG 310 320 330 340 350 360 VLSAKGDFSA GDIIEVFFNN RPIGKGKTNF SKHELELIKG LNSEEALSLT KRSKKAVIHR 370 DFWVATKKEC SY

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64302.1.
RefSeq	YP_175263.1.
3D structure databases
SMR	Q5WH53. Positions 3-365.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WH53.
Genome annotation databases
GeneID	3202778.
GenomeReviews	Gene locus ABC1767 in contig AP006627_GR.
KEGG	bcl:ABC1767.
NMPDR	fig|66692.3.peg.1715.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TDVDRLY.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC1767-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BACSK

Accession

Primary (citable) accession number: Q5WH53

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)