Reviewed, UniProtKB/Swiss-Prot Q5WH11 (IOLA2_BACSK)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 2
      Short name=MMSA dehydrogenase 2
      Short name=MMSDH 2
      Short name=MSDH 2
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 2
      Short name=MSA dehydrogenase 2
    EC=1.2.1.18

Gene names

Name:	iolA2
Ordered Locus Names:	ABC1809

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670		PRO_0000352327
Regions
	Nucleotide binding	178 – 182	5	NAD By similarity
Sites
	Active site	286	1	Nucleophile By similarity
	Binding site	385	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WH11-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DA534B62243E3144	FASTA	486	52,752
	10 20 30 40 50 60 MTTTKVKTVK NWIDGAWVEA STADTEVVPN PATGEAIAYV PLSGERDVEQ AVASAKRAYE 70 80 90 100 110 120 TWKTVPVPER TRYMFAYLEQ LKKNREQLAQ LITLENGKTI KDARGEVQRG IECVELATST 130 140 150 160 170 180 PTMMMGDALP DIASGIDGSI WRYPLGVVAG ITPFNFPMMV PLWMFPLAIA AGNTFVLKTS 190 200 210 220 230 240 ERTPLLAEQL VSLMHEVGLP RGVLNLVNGG KAVVNGLLNH PDVEAISFVG SEPVARYVYE 250 260 270 280 290 300 TGTANGKRVQ ALAGAKNHAV VLADCELDKT VQGVIGAAFA SSGERCMACS VVAVVEEVAD 310 320 330 340 350 360 AFIEKLTAET KKLTVGNGKN DEHFVGPLIR QSHKDKVVKY IEQGVEQGAE LLVDGRNATS 370 380 390 400 410 420 EEAGYYLGAT LFDHVTPEMT IWQEELFAPV LSIVRVRDLE EAIALTNRSR FANGAVIYTS 430 440 450 460 470 480 SGKAAQHFRN AIDAGMVGIN VNVPAPMAFF SFAGNKASFF GDLGTNGRDG IQFYTRKKVV TERWFN

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64344.1.
RefSeq	YP_175305.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3202963.
GenomeReviews	Gene locus ABC1809 in contig AP006627_GR.
KEGG	bcl:ABC1809.
NMPDR	fig|66692.3.peg.1757.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WH11.
OMA	Q5WH11. KVGPGNG.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA2_BACSK

Accession

Primary (citable) accession number: Q5WH11

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)