Reviewed, UniProtKB/Swiss-Prot Q5WGB1 (SYN_BACSK)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	ABC2059

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176392

Sequences

	Sequence		Length	Mass (Da)
	Q5WGB1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 807913F5DCC4DE99	FASTA	430	49,077
	10 20 30 40 50 60 MKTTIAQVGR YVNEQVTIGA WIANKRSSGK IAFLQLRDGT GFIQGVVVKA EVPEDVFALA 70 80 90 100 110 120 KSITQESSLY VTGTVREDER SPSGYELSVE QIELIHQATD YPITPKQHGT EFLMDHRHLW 130 140 150 160 170 180 LRSKRQHAIM KIRSQIIRST YDFFSERGFT KLDSPILTGS SPEGTSELFH TKYFDEDAYL 190 200 210 220 230 240 SQSGQLYAEA GAMALGRVFT FGPTFRAEKS KTRRHLIEFW MIEPEMAFVE HEESLEIQEQ 250 260 270 280 290 300 YVAHLAKAVL DHCQLELKQL GRDVTKLEAI KAPFPRITYD EALDLLKEKG FDDISWGDDF 310 320 330 340 350 360 GSPHETAIAE HFDKPVFITR YPRSLKPFYM QPAPDRDDVV LCADLIAPEG YGEIIGGSER 370 380 390 400 410 420 IHDYDLLKQE LEKHNLPLDA YGWYLDLRKY GSVPHSGFGL GLERTVAWLS GTEHVRETIP 430 FPRLLNRLYP

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64594.1.
RefSeq	YP_175555.1.
3D structure databases
SMR	Q5WGB1. Positions 11-430.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WGB1.
Genome annotation databases
GeneID	3201735.
GenomeReviews	Gene locus ABC2059 in contig AP006627_GR.
KEGG	bcl:ABC2059.
NMPDR	fig|66692.3.peg.2363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2059-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACSK

Accession

Primary (citable) accession number: Q5WGB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)