Reviewed, UniProtKB/Swiss-Prot Q5WG44 (QUEF_BACSK)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	ABC2126

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000162954

Sequences

	Sequence		Length	Mass (Da)
	Q5WG44-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 2ADAD4A73579A0CF	FASTA	165	19,531
	10 20 30 40 50 60 MAGRQEKELQ NVTLLGSEQT EYKYSYDPRV LETFDNQHPY RDYMVKFNCP EFTTLCPKTG 70 80 90 100 110 120 QPDFATLYIS YIPEQKMVES KSLKLYLFSF RNHGDFHEDS VNTIMNDLIE LMDPRYIEVW 130 140 150 160 GKFTPRGGIS IDPYCNYGKK GTKYEEIANY RLMNHDLYPE TITNR

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64661.1.
RefSeq	YP_175622.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3203664.
GenomeReviews	Gene locus ABC2126 in contig AP006627_GR.
KEGG	bcl:ABC2126.
NMPDR	fig|66692.3.peg.2430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WG44.
OMA	Q5WG44. HESCINT.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2126-MON.
Family and domain databases
HAMAP	MF_00818. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
ProDom	PD003330. GTP_cyclohydroI. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BACSK

Accession

Primary (citable) accession number: Q5WG44

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)