Reviewed, UniProtKB/Swiss-Prot Q5WG03 (LEXA_BACSK)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	ABC2167

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	LexA repressor HAMAP MF_00015		PRO_0000170009
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	130	1	For autocatalytic cleavage activity By similarity
	Active site	168	1	For autocatalytic cleavage activity By similarity
	Site	95 – 96	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WG03-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4C967CAB4A10F24B	FASTA	208	23,179
	10 20 30 40 50 60 MSKLSKRQEE ILAYIKDEVK KKGYPPSVRE IGEAVGLASS STVHGHLARL EKKGYIRRDP 70 80 90 100 110 120 TKPRAIEVLS LGFDNDTFVK KETASFIPVI GKVTAGVPIT AVENVEDYLP LPDHLAAYDN 130 140 150 160 170 180 TYALVIQGES MIEAGIYDGD QVIVRQQQTA DNGDIIVAMT EDNEATVKRF FREKDYIRLQ 190 200 PENSSMEPII LENCTILGKV IGVFRTIH

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64702.1.
RefSeq	YP_175663.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3204066.
GenomeReviews	Gene locus ABC2167 in contig AP006627_GR.
KEGG	bcl:ABC2167.
NMPDR	fig|66692.3.peg.2471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WG03.
OMA	Q5WG03. KVIGVFR.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2167-MON.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BACSK

Accession

Primary (citable) accession number: Q5WG03

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)