Reviewed, UniProtKB/Swiss-Prot Q5WFT6 (SYP_BACSK)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	ABC2234

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248646

Sequences

	Sequence		Length	Mass (Da)
	Q5WFT6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 49C6C3D39620C995	FASTA	566	62,558
	10 20 30 40 50 60 MRQSTYLAPT MRDVPADAEA ISHQLMLRAG MMRQIAAGVY AYLPLAKRTI AKIEAIIREE 70 80 90 100 110 120 LDAIGAQELT LPSLHPAELW QASGRWEAMG DELVRLKDRH NRDFALGPTH EEVITSLIKD 130 140 150 160 170 180 GIASYKKLPL CVYQVQTKFR DERRPRFGLL RGREFIMKDA YSFHDSPESL DEAYWQMHGA 190 200 210 220 230 240 YSRIFTRVGL EFRPVIADSG AMGGKDTHEF MALASVGEDT VVYSDGSDYA ANLEMAKAAL 250 260 270 280 290 300 PEAPSVQAHG LLEKRATPEA KTVDEAAEAL GFDADDIFKA LVVVVDDVLS LFILRGNDEL 310 320 330 340 350 360 NEVKALHELG AKTLRMASEE EVVAAFGATP GSIGPVGVKD VPIYADYRIR SMERIACGAN 370 380 390 400 410 420 ETGYHYVNVG PGRDYEVTAY KDLRTVREGD PSPDGKGTLR FAEGIEIGQI FKLGTRYSES 430 440 450 460 470 480 LDAKYLDGQG KAQPFLMGCY GIGVSRTLAA VIEQHHDEAG IVWPKAVAPF DVHLLALNVK 490 500 510 520 530 540 NEDQKTLAEQ LYSDIRQAGI DVLYDDRPER AGVKFKDADL IGLPVRVAVG KRAGEGIIEV 550 560 KVRKTGEQLE LTASELVRWL NDFLRE

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64769.1.
RefSeq	YP_175730.1.
3D structure databases
HSSP	HSSP built from PDB template 1DBU based on UniProtKB P45202.
SMR	Q5WFT6. Positions 1-564.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFT6.
Genome annotation databases
GeneID	3202780.
GenomeReviews	Gene locus ABC2234 in contig AP006627_GR.
KEGG	bcl:ABC2234.
NMPDR	fig|66692.3.peg.2538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2234-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACSK

Accession

Primary (citable) accession number: Q5WFT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)