Reviewed, UniProtKB/Swiss-Prot Q5WFT4 (DXR_BACSK)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	ABC2236

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163607
Regions
	Nucleotide binding	7 – 36	30	NADP By similarity
Sites
	Metal binding	147	1	Divalent metal cation By similarity
	Metal binding	149	1	Divalent metal cation By similarity
	Metal binding	218	1	Divalent metal cation By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	218	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WFT4-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: FB2662ECA440D873	FASTA	379	41,581
	10 20 30 40 50 60 MKQISLLGST GSIGTQTLDV IRDYPDQFTL SALACGTNLE LCRNQIKEFQ PKLVSVQRPE 70 80 90 100 110 120 DAQTLAGEFG DSIEFLYGEE GLKEVARYSE SDIVVTAITG SIGLLPTLSA IKAKKAVAIA 130 140 150 160 170 180 NKETLVSAGH LVVSAAKEND VPLIPVDSEH SAIFQALNGE PKKAVDRLIL TASGGSFRDK 190 200 210 220 230 240 SRAELDGVTV EQALAHPNWS MGAKVTIDSA TMMNKGLEVI EAKWLFGLDY DQIDVLIHKE 250 260 270 280 290 300 SIIHSMVEYV DRSVIAQLGT PDMRVPIQYA LTYPERFARP KHERLDLAEL GKLHFEKMDM 310 320 330 340 350 360 ERFRCMKLAY EAGRAGGTMT TVLNAANEVA VCRFLQGEIT FLEIERIIEE ALSAHSPIAH 370 PSLEEIQAVD ASIRKQLQA

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64771.1.
RefSeq	YP_175732.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFT4.
Genome annotation databases
GeneID	3202338.
GenomeReviews	Gene locus ABC2236 in contig AP006627_GR.
KEGG	bcl:ABC2236.
NMPDR	fig|66692.3.peg.2540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WFT4.
OMA	IHSMVEY.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2236-MON.
Family and domain databases
HAMAP	MF_00183. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_BACSK

Accession

Primary (citable) accession number: Q5WFT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)