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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:ABC2277
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001172311 – 436Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST436

Interactioni

Protein-protein interaction databases

STRINGi66692.ABC2277.

Structurei

3D structure databases

ProteinModelPortaliQ5WFP8.
SMRiQ5WFP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5WFP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHKQSINVI GAGLAGSEAA WQIAKRGGHV RLYEMRPVKQ TPAHHTDKFA
60 70 80 90 100
ELVCSNSLRG NSLANAVGVL KEEMRHLDSV IIKAADEAAV PAGGALAVDR
110 120 130 140 150
HDFAGKVTEY VKGHPNVTVV QEEVTEIPEG PAIIATGPLT SEALAEQLKA
160 170 180 190 200
FSGEEYLYFY DAAAPIIDAE TIDRDKVYLK SRYDKGEAAY LNCPMTEEEF
210 220 230 240 250
DRFYQALIEA ETVPLREFEK DIFFEGCMPI EVMASRGKKT MLFGPLKPVG
260 270 280 290 300
LEDPKTGKRP FAVVQLRQDN SSGTLYNMVG FQTHLKWGPQ KEVIRMIPGL
310 320 330 340 350
ENADIVRYGV MHRNTFLNSP NLLKPTYQSK KRSDLFFAGQ MTGVEGYVES
360 370 380 390 400
AAAGLVAGIN AFKWTQSQEL AVFPEETMIG SMAAYITNAN AKTFQPMNAN
410 420 430
FGLVPPLNVR IKAKKERYEA LAKRALESIQ NFMKEV
Length:436
Mass (Da):48,231
Last modified:November 23, 2004 - v1
Checksum:i89CA481A0C744737
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD64812.1.
RefSeqiWP_011247120.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD64812; BAD64812; ABC2277.
KEGGibcl:ABC2277.
PATRICi18924134. VBIBacCla58185_2430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD64812.1.
RefSeqiWP_011247120.1. NC_006582.1.

3D structure databases

ProteinModelPortaliQ5WFP8.
SMRiQ5WFP8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi66692.ABC2277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD64812; BAD64812; ABC2277.
KEGGibcl:ABC2277.
PATRICi18924134. VBIBacCla58185_2430.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_BACSK
AccessioniPrimary (citable) accession number: Q5WFP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 23, 2004
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.