Q5WFP7 (Q5WFP7_BACSK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAD64813.1
Ordered Locus Names:	ABC2278

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5WFP7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DD295A925791B50A	FASTA	690	79,106
	10 20 30 40 50 60 MSDYLVIVES PAKAKTIGKY LGKKYVVKAS MGHVRDLPKS QMGVSVEDNY EPRYITIRGK 70 80 90 100 110 120 GPVLKELKTA AKKAKRVYLA ADPDREGEAI AWHLAHSLNI DENSDCRVVF NEITKTAIKD 130 140 150 160 170 180 AFKHPRPINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVKLITDREK 190 200 210 220 230 240 EIQQFVPEEY WSITGTFLLG DEPFEAKFYG RNGKKESLTS EENVNEVLKQ IKGKQFAVAD 250 260 270 280 290 300 VVKKERRRNP VSPFTTSSLQ QEAARKLNFR AKKTMLIAQQ LYEGIDLGKE GTVGLITYMR 310 320 330 340 350 360 TDSTRISDTA KQETYEYIRS TYGEEYARKG ERKQKQDKNA QDAHEAIRPT SVKYEPKQLK 370 380 390 400 410 420 EYLSRDQLRL YRLIWERLVA SEMAPAIMDT MTVDLENNGV KFRANGSKVK FPGFMKVYIE 430 440 450 460 470 480 GNDDNKKEED RLLPALAKGQ QVEKTEVTPN QHFTQPPPRY TEARLVKTLE ELGIGRPSTY 490 500 510 520 530 540 APTLDTIQRR GYVALDEKRF VPTELGEIVL DLIVEFFPEI LDVEFTAKME EDLDSIEDGR 550 560 570 580 590 600 DNWISIIDQF YQSFEKRLKV AEEEMEEVEI KDEPAGEDCE KCGHEMVYKM GRYGKFMACS 610 620 630 640 650 660 NFPECRNTKA IVKEIGVTCP TCKQGNIVER KSKKRRIFYG CDRYPDCEFV SWDKPISRPC 670 680 690 PKCQGLLVEK KSKKGVFVAC TSCDYEEEKQ

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64813.1.
RefSeq	YP_175774.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WFP7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000047224; EBBACP00000045985; EBBACG00000047215.
GeneID	3203428.
GenomeReviews	Gene locus ABC2278 in contig AP006627_GR.
KEGG	bcl:ABC2278.
NMPDR	fig|66692.3.peg.1966.
PATRIC	18924136. VBIBacCla58185_2431.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q5WFP7_BACSK

Accession

Primary (citable) accession number: Q5WFP7

Entry history

Integrated into UniProtKB/TrEMBL:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)