Reviewed, UniProtKB/Swiss-Prot Q5WFM6 (RNC_BACSK)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	ABC2299

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Ribonuclease 3 HAMAP MF_00104		PRO_0000228493
Regions
	Domain	33 – 162	130	RNase III
	Domain	188 – 257	70	DRBM

Sequences

	Sequence		Length	Mass (Da)
	Q5WFM6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4E8AD19A27ABA7E1	FASTA	260	29,878
	10 20 30 40 50 60 MAQSSKYQRK PRSGERKRSQ RRLELTEEQK KQFDDLLVRT GLTFENRKLL IQAFTHSSYV 70 80 90 100 110 120 NEQRIFSSSD NERLEFLGDA VLELAVSQYL FKTYKNMSEG DMTKLRASIV CEPSLARFAE 130 140 150 160 170 180 ELQFGKLVLL GKGEEVTGGR TRPALLADVF EAFIGALYMD QGLEPVFMFL ARFMYPKIKE 190 200 210 220 230 240 GAFTYKMDFK SQLQEFVQRD NRGQIRYLIV QERGPAHDRE FVSDVQLNEE TIGTGTGRSK 250 260 KEAEQLAAKQ ALLALNQKES

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64834.1.
RefSeq	YP_175795.1.
3D structure databases
SMR	Q5WFM6. Positions 25-255.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFM6.
Genome annotation databases
GeneID	3201517.
GenomeReviews	Gene locus ABC2299 in contig AP006627_GR.
KEGG	bcl:ABC2299.
NMPDR	fig|66692.3.peg.1987.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HBG744556.
OMA	ALTHKSF.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2299-MONOMER.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BACSK

Accession

Primary (citable) accession number: Q5WFM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)