Q5WFJ6 (Q5WFJ6_BACSK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase HAMAP MF_01208
Short name=OPRT HAMAP MF_01208
Short name=OPRTase HAMAP MF_01208
EC=2.4.2.10 HAMAP MF_01208

Gene names

Name:	pyrE HAMAP MF_01208 EMBL BAD64864.1
Ordered Locus Names:	ABC2329

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208 SAAS SAAS004467
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208 SAAS SAAS023031
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208 SAAS SAAS023031
Subunit structure	Homodimer By similarity. HAMAP MF_01208 SAAS SAAS004467
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. HAMAP MF_01208

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis SAAS SAAS023031 HAMAP MF_01208
Ligand	Magnesium HAMAP MF_01208
Molecular function	Glycosyltransferase HAMAP MF_01208 SAAS SAAS023031 EMBL BAD64864.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	128 – 136	9	5-phosphoribose 1-diphosphate binding By similarity HAMAP MF_01208
Sites
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	106	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	108	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	132	1	Orotate By similarity HAMAP MF_01208

Sequences

	Sequence		Length	Mass (Da)
	Q5WFJ6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 567A0A474E6EC63B	FASTA	218	23,450
	10 20 30 40 50 60 MERGDRTLTS RTIANHLLNI KAVTLSPAKP YTWSSGLKSP IYCDNRLTLS YPHIRNEIAE 70 80 90 100 110 120 GLVAMIREAA PDADIIAGTA TAGIPHAAFV AEKLQLPMIY VRSSAKGHGK QNAIEGHLPS 130 140 150 160 170 180 GSKVVVIEDL ISTGGSSIAA AEALRAAGAE VLLVAAIFSY QLSQSDENFN GAKLPVRVLT 190 200 210 TYATLVEEAQ KRGAITSEEQ AKLEAWRKNP KDEAWMNL

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64864.1.
RefSeq	YP_175825.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WFJ6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFJ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000048242; EBBACP00000047003; EBBACG00000048233.
GeneID	3202828.
GenomeReviews	Gene locus ABC2329 in contig AP006627_GR.
KEGG	bcl:ABC2329.
NMPDR	fig|66692.3.peg.2017.
PATRIC	18924246. VBIBacCla58185_2486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
GeneTree	EBGT00050000002725.
HOGENOM	HBG404341.
OMA	LPMTYVR.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q5WFJ6_BACSK

Accession

Primary (citable) accession number: Q5WFJ6

Entry history

Integrated into UniProtKB/TrEMBL:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)