Reviewed, UniProtKB/Swiss-Prot Q5WFJ4 (PYRD_BACSK)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	ABC2331

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00224
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (O2 route): step 1/1. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 pyrK and 2 pyrD subunits By similarity. HAMAP MF_00224
Subcellular location	Cytoplasm By similarity HAMAP MF_00224.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Dihydroorotate dehydrogenase HAMAP MF_00224		PRO_1000024130
Sites
	Active site	130	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WFJ4-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 795EFBE0FF5E488A	FASTA	304	32,447
	10 20 30 40 50 60 MERLQISLPG LEMKNPVMPA SGCFGFGKEY GQFFDLNQLG AIAIKATTKE ARFGNETPRV 70 80 90 100 110 120 AETKAGMLNA IGLQNPGLQG VIEQELPRFD DYDVPILANI AGSTMDDYIE VAAELSQQRN 130 140 150 160 170 180 VSAIELNISC PNVKQGGIAF GTVPEIAAEL TKEVKAVSAV PVYVKLSPNV ADIVAIAKAI 190 200 210 220 230 240 EKAGADGLAM INTLLGMRID AKTRRPVLAN QYGGLSGAAI KPVALRMIHQ VSQQVDIPII 250 260 270 280 290 300 GMGGIQTAED VIEFLLAGAS CIAVGTANFT DPYTCPNIIR DLPHWLDRLE VQSITDLIGG SWNE

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD64866.1.
RefSeq	YP_175827.1.
3D structure databases
HSSP	HSSP built from PDB template 1EP2 based on UniProtKB P54322.
SMR	Q5WFJ4. Positions 3-298.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFJ4.
Genome annotation databases
GeneID	3202826.
GenomeReviews	Gene locus ABC2331 in contig AP006627_GR.
KEGG	bcl:ABC2331.
NMPDR	fig|66692.3.peg.2019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG472415.
OMA	MPASGCF.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2331-MONOMER.
Family and domain databases
HAMAP	MF_00224_B. DHO_dh_type1_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BACSK

Accession

Primary (citable) accession number: Q5WFJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)