Reviewed, UniProtKB/Swiss-Prot Q5WFJ0 (PYRC_BACSK)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	ABC2335

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Dihydroorotase		PRO_0000325587
Sites
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	139	1	Zinc 1; via carbamate group By similarity
	Metal binding	139	1	Zinc 2; via carbamate group By similarity
	Metal binding	176	1	Zinc 2 By similarity
	Metal binding	229	1	Zinc 2 By similarity
	Metal binding	302	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	139	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WFJ0-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C207AABD1D32A4CC	FASTA	427	45,862
	10 20 30 40 50 60 MAMKVTGGFI LDENGEQVAK DVYIKDGKIV EHVADGDIRQ QFDATGLLIS PGFVDVHVHL 70 80 90 100 110 120 REPGGEKKET IETGTKAAAR GGFTTVAAMP NTRPVPDNAE QLDLLQARIS ETAVVRVLPY 130 140 150 160 170 180 ASITTRQLGQ ELTDFKALKE AGAFAFTDDG VGIQEAGMML SAMKEAAALN MAVVAHCEDN 190 200 210 220 230 240 SLINGGAVHE GHYAKAHGLN GIPSVCEAVH IARDVLLAEA AGAHYHVCHV STKESVRTIR 250 260 270 280 290 300 DAKKAGIRVT AEVTPHHLLL CEDDIIGKDP NFKMNPPLRA KEDRDALVAG LLDGTIDFIA 310 320 330 340 350 360 TDHAPHTAEE KSASLERAPF GIVGLETAFP LLYTHFVKPG TFTLKQLIDW LTVKPAQTFN 370 380 390 400 410 420 LPYGTLQVGA AADLTLIDLK ANETIDPSMF LSKGKNTPFA GWDCAGIPQA TMVAGKTVYK KERITNE

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64870.1.
RefSeq	YP_175831.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3202825.
GenomeReviews	Gene locus ABC2335 in contig AP006627_GR.
KEGG	bcl:ABC2335.
NMPDR	fig|66692.3.peg.2023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WFJ0.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2335-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_BACSK

Accession

Primary (citable) accession number: Q5WFJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	November 23, 2004
Last modified:	September 2, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)