Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5WFI3

- SYI_BACSK

UniProt

Q5WFI3 - SYI_BACSK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-2410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ABC2342
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001168: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Isoleucine--tRNA ligasePRO_0000098348Add
BLAST

Proteomic databases

PRIDEiQ5WFI3.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC2342.

Structurei

3D structure databases

ProteinModelPortaliQ5WFI3.
SMRiQ5WFI3. Positions 2-878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5WFI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKQTLLMP KTAFPMRGNL PNSEPKRQEQ WEEMDIYKQV QERTEGRPYF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KIIKDFIVRY KSMSGFHAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VDRKSMSIAD FRKLCEEYAL EQINSQRQQF MRLGVRGDWW
160 170 180 190 200
KPYITLEKEF EAEQIKVFGK MAKKGYIYKG KKPVYWSPSS ESALAEAEIE
210 220 230 240 250
YYDKRSASIY VAFQVADGKG VLPEDAALVI WTTTPWTIPA NLGITVHPQL
260 270 280 290 300
EYSVVRTGGR SFVVASGLLE HVQTELSWED VVVEKTVTGK ELDYIVCRHP
310 320 330 340 350
FYDRDSIVMN GEHVTLDAGT GCVHTAPGHG VDDFVVGQKY GLDVLCPIDD
360 370 380 390 400
KGIMTSEAPG FEGEFYDNIN KMVTEKLEAV GALLKLGFIT HSYPHDWRTK
410 420 430 440 450
KPVIFRATSQ WFASIANIRK ELLQAVANTE WIPAWGETRL HNMVRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYGEDG TEIVSEETIE HVSNLFREHG SNVWFEREAK
510 520 530 540 550
DLLPPGFTSP HSPNGHFTKE TDIMDVWFDS GSSHQGVLRE RNDLVFPADL
560 570 580 590 600
YFEGSDQYRG WFNSSLSTSV AINGVAPYKG VLSHGFVMDG EGKKMSKSLG
610 620 630 640 650
NIVVPNTVMK QLGADILRLW VASVDYQADS RVSDSILKQV AETYRKIRNT
660 670 680 690 700
FRFLLGNLHD FDPKINAVPR DQLTGVHAFL LVRLNELTKR MHEGYNEYQF
710 720 730 740 750
LNVYNHFRNF CTVELSSFYM DISKDTLYIE HADHPERRAF QTVMYEVVTA
760 770 780 790 800
LTKLIAPILP HTADEVWENI PGVSEQSVQL TDMPTAEELS GTDELKAHWQ
810 820 830 840 850
AFMDIRADVL KALEVARNEK VIGKSLQASL TLYPNKQARA LLEATEGLKK
860 870 880 890 900
LFIVSNVQIA NEGDEVPAAA QQFADVAVLV EKAEGETCER CWQVSNTVGA
910 920
DEKHPTLCLS CAQTVTNHYN DVV
Length:923
Mass (Da):104,537
Last modified:November 23, 2004 - v1
Checksum:iE25EFE72971B29FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD64877.1.
RefSeqiYP_175838.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD64877; BAD64877; ABC2342.
GeneIDi3201308.
KEGGibcl:ABC2342.
PATRICi18924272. VBIBacCla58185_2499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD64877.1 .
RefSeqi YP_175838.1. NC_006582.1.

3D structure databases

ProteinModelPortali Q5WFI3.
SMRi Q5WFI3. Positions 2-878.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 66692.ABC2342.

Proteomic databases

PRIDEi Q5WFI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD64877 ; BAD64877 ; ABC2342 .
GeneIDi 3201308.
KEGGi bcl:ABC2342.
PATRICi 18924272. VBIBacCla58185_2499.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BCLA66692:GHMP-2410-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
    Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KSM-K16.

Entry informationi

Entry nameiSYI_BACSK
AccessioniPrimary (citable) accession number: Q5WFI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3