Reviewed, UniProtKB/Swiss-Prot Q5WFI3 (SYI_BACSK)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	ABC2342

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_0000098348
Regions
	Motif	57 – 67	11	"HIGH" region HAMAP MF_02002
	Motif	594 – 598	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	888	1	Zinc By similarity
	Metal binding	891	1	Zinc By similarity
	Metal binding	908	1	Zinc By similarity
	Metal binding	911	1	Zinc By similarity
	Binding site	553	1	Aminoacyl-adenylate By similarity
	Binding site	597	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WFI3-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E25EFE72971B29FD	FASTA	923	104,537
	10 20 30 40 50 60 MDYKQTLLMP KTAFPMRGNL PNSEPKRQEQ WEEMDIYKQV QERTEGRPYF VLHDGPPYAN 70 80 90 100 110 120 GDIHMGHALN KIIKDFIVRY KSMSGFHAPY VPGWDTHGLP IETALTKNKK VDRKSMSIAD 130 140 150 160 170 180 FRKLCEEYAL EQINSQRQQF MRLGVRGDWW KPYITLEKEF EAEQIKVFGK MAKKGYIYKG 190 200 210 220 230 240 KKPVYWSPSS ESALAEAEIE YYDKRSASIY VAFQVADGKG VLPEDAALVI WTTTPWTIPA 250 260 270 280 290 300 NLGITVHPQL EYSVVRTGGR SFVVASGLLE HVQTELSWED VVVEKTVTGK ELDYIVCRHP 310 320 330 340 350 360 FYDRDSIVMN GEHVTLDAGT GCVHTAPGHG VDDFVVGQKY GLDVLCPIDD KGIMTSEAPG 370 380 390 400 410 420 FEGEFYDNIN KMVTEKLEAV GALLKLGFIT HSYPHDWRTK KPVIFRATSQ WFASIANIRK 430 440 450 460 470 480 ELLQAVANTE WIPAWGETRL HNMVRDRGDW CISRQRAWGV PIPVFYGEDG TEIVSEETIE 490 500 510 520 530 540 HVSNLFREHG SNVWFEREAK DLLPPGFTSP HSPNGHFTKE TDIMDVWFDS GSSHQGVLRE 550 560 570 580 590 600 RNDLVFPADL YFEGSDQYRG WFNSSLSTSV AINGVAPYKG VLSHGFVMDG EGKKMSKSLG 610 620 630 640 650 660 NIVVPNTVMK QLGADILRLW VASVDYQADS RVSDSILKQV AETYRKIRNT FRFLLGNLHD 670 680 690 700 710 720 FDPKINAVPR DQLTGVHAFL LVRLNELTKR MHEGYNEYQF LNVYNHFRNF CTVELSSFYM 730 740 750 760 770 780 DISKDTLYIE HADHPERRAF QTVMYEVVTA LTKLIAPILP HTADEVWENI PGVSEQSVQL 790 800 810 820 830 840 TDMPTAEELS GTDELKAHWQ AFMDIRADVL KALEVARNEK VIGKSLQASL TLYPNKQARA 850 860 870 880 890 900 LLEATEGLKK LFIVSNVQIA NEGDEVPAAA QQFADVAVLV EKAEGETCER CWQVSNTVGA 910 920 DEKHPTLCLS CAQTVTNHYN DVV

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64877.1.
RefSeq	YP_175838.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WFI3.
Genome annotation databases
GeneID	3201308.
GenomeReviews	Gene locus ABC2342 in contig AP006627_GR.
KEGG	bcl:ABC2342.
NMPDR	fig|66692.3.peg.2030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WFI3.
OMA	FPMRGNL.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2342-MON.
Family and domain databases
HAMAP	MF_02002. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BACSK

Accession

Primary (citable) accession number: Q5WFI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)