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Q5WFI3

- SYI_BACSK

UniProt

Q5WFI3 - SYI_BACSK

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Protein
Isoleucine--tRNA ligase
Gene
ileS, ABC2342
Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-2410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:ABC2342
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001168: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098348Add
BLAST

Proteomic databases

PRIDEiQ5WFI3.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC2342.

Structurei

3D structure databases

ProteinModelPortaliQ5WFI3.
SMRiQ5WFI3. Positions 2-878.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5WFI3-1 [UniParc]FASTAAdd to Basket

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MDYKQTLLMP KTAFPMRGNL PNSEPKRQEQ WEEMDIYKQV QERTEGRPYF    50
VLHDGPPYAN GDIHMGHALN KIIKDFIVRY KSMSGFHAPY VPGWDTHGLP 100
IETALTKNKK VDRKSMSIAD FRKLCEEYAL EQINSQRQQF MRLGVRGDWW 150
KPYITLEKEF EAEQIKVFGK MAKKGYIYKG KKPVYWSPSS ESALAEAEIE 200
YYDKRSASIY VAFQVADGKG VLPEDAALVI WTTTPWTIPA NLGITVHPQL 250
EYSVVRTGGR SFVVASGLLE HVQTELSWED VVVEKTVTGK ELDYIVCRHP 300
FYDRDSIVMN GEHVTLDAGT GCVHTAPGHG VDDFVVGQKY GLDVLCPIDD 350
KGIMTSEAPG FEGEFYDNIN KMVTEKLEAV GALLKLGFIT HSYPHDWRTK 400
KPVIFRATSQ WFASIANIRK ELLQAVANTE WIPAWGETRL HNMVRDRGDW 450
CISRQRAWGV PIPVFYGEDG TEIVSEETIE HVSNLFREHG SNVWFEREAK 500
DLLPPGFTSP HSPNGHFTKE TDIMDVWFDS GSSHQGVLRE RNDLVFPADL 550
YFEGSDQYRG WFNSSLSTSV AINGVAPYKG VLSHGFVMDG EGKKMSKSLG 600
NIVVPNTVMK QLGADILRLW VASVDYQADS RVSDSILKQV AETYRKIRNT 650
FRFLLGNLHD FDPKINAVPR DQLTGVHAFL LVRLNELTKR MHEGYNEYQF 700
LNVYNHFRNF CTVELSSFYM DISKDTLYIE HADHPERRAF QTVMYEVVTA 750
LTKLIAPILP HTADEVWENI PGVSEQSVQL TDMPTAEELS GTDELKAHWQ 800
AFMDIRADVL KALEVARNEK VIGKSLQASL TLYPNKQARA LLEATEGLKK 850
LFIVSNVQIA NEGDEVPAAA QQFADVAVLV EKAEGETCER CWQVSNTVGA 900
DEKHPTLCLS CAQTVTNHYN DVV 923
Length:923
Mass (Da):104,537
Last modified:November 23, 2004 - v1
Checksum:iE25EFE72971B29FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD64877.1.
RefSeqiYP_175838.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD64877; BAD64877; ABC2342.
GeneIDi3201308.
KEGGibcl:ABC2342.
PATRICi18924272. VBIBacCla58185_2499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD64877.1 .
RefSeqi YP_175838.1. NC_006582.1.

3D structure databases

ProteinModelPortali Q5WFI3.
SMRi Q5WFI3. Positions 2-878.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 66692.ABC2342.

Proteomic databases

PRIDEi Q5WFI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD64877 ; BAD64877 ; ABC2342 .
GeneIDi 3201308.
KEGGi bcl:ABC2342.
PATRICi 18924272. VBIBacCla58185_2499.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BCLA66692:GHMP-2410-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
    Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KSM-K16.

Entry informationi

Entry nameiSYI_BACSK
AccessioniPrimary (citable) accession number: Q5WFI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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