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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi888ZincUniRule annotation1
Metal bindingi891ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCLA66692:G1G25-2480-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ABC2342
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983481 – 923Isoleucine--tRNA ligaseAdd BLAST923

Proteomic databases

PRIDEiQ5WFI3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC2342

Structurei

3D structure databases

ProteinModelPortaliQ5WFI3
SMRiQ5WFI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q5WFI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKQTLLMP KTAFPMRGNL PNSEPKRQEQ WEEMDIYKQV QERTEGRPYF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KIIKDFIVRY KSMSGFHAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VDRKSMSIAD FRKLCEEYAL EQINSQRQQF MRLGVRGDWW
160 170 180 190 200
KPYITLEKEF EAEQIKVFGK MAKKGYIYKG KKPVYWSPSS ESALAEAEIE
210 220 230 240 250
YYDKRSASIY VAFQVADGKG VLPEDAALVI WTTTPWTIPA NLGITVHPQL
260 270 280 290 300
EYSVVRTGGR SFVVASGLLE HVQTELSWED VVVEKTVTGK ELDYIVCRHP
310 320 330 340 350
FYDRDSIVMN GEHVTLDAGT GCVHTAPGHG VDDFVVGQKY GLDVLCPIDD
360 370 380 390 400
KGIMTSEAPG FEGEFYDNIN KMVTEKLEAV GALLKLGFIT HSYPHDWRTK
410 420 430 440 450
KPVIFRATSQ WFASIANIRK ELLQAVANTE WIPAWGETRL HNMVRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYGEDG TEIVSEETIE HVSNLFREHG SNVWFEREAK
510 520 530 540 550
DLLPPGFTSP HSPNGHFTKE TDIMDVWFDS GSSHQGVLRE RNDLVFPADL
560 570 580 590 600
YFEGSDQYRG WFNSSLSTSV AINGVAPYKG VLSHGFVMDG EGKKMSKSLG
610 620 630 640 650
NIVVPNTVMK QLGADILRLW VASVDYQADS RVSDSILKQV AETYRKIRNT
660 670 680 690 700
FRFLLGNLHD FDPKINAVPR DQLTGVHAFL LVRLNELTKR MHEGYNEYQF
710 720 730 740 750
LNVYNHFRNF CTVELSSFYM DISKDTLYIE HADHPERRAF QTVMYEVVTA
760 770 780 790 800
LTKLIAPILP HTADEVWENI PGVSEQSVQL TDMPTAEELS GTDELKAHWQ
810 820 830 840 850
AFMDIRADVL KALEVARNEK VIGKSLQASL TLYPNKQARA LLEATEGLKK
860 870 880 890 900
LFIVSNVQIA NEGDEVPAAA QQFADVAVLV EKAEGETCER CWQVSNTVGA
910 920
DEKHPTLCLS CAQTVTNHYN DVV
Length:923
Mass (Da):104,537
Last modified:November 23, 2004 - v1
Checksum:iE25EFE72971B29FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA Translation: BAD64877.1
RefSeqiWP_011247185.1, NC_006582.1

Genome annotation databases

EnsemblBacteriaiBAD64877; BAD64877; ABC2342
GeneIDi34043932
KEGGibcl:ABC2342

Similar proteinsi

Entry informationi

Entry nameiSYI_BACSK
AccessioniPrimary (citable) accession number: Q5WFI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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